Amplification of fluorescent DNA through enzymatic incorporation of a highly emissive deoxyguanosine analogue

A highly emissive thio-analogue of deoxyguanosine triphosphate, super(th)dGTP, was synthesized and enzymatically incorporated into DNA through primer extension and PCR amplification. The straightforward amplification of super(th)dG-labeled DNA by natural polymerases and simple addition of super(th)d...

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Bibliographic Details
Published inRSC advances Vol. 4; no. 59; pp. 31341 - 31344
Main Authors Otomo, Haruka, Park, Soyoung, Yamamoto, Seigi, Sugiyama, Hiroshi
Format Journal Article
LanguageEnglish
Published 01.01.2014
Subjects
Addition polymerization
Amplification
Analogue
Deoxyribonucleic acid
Emissivity
Fluorescence
Synthesis
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Summary:A highly emissive thio-analogue of deoxyguanosine triphosphate, super(th)dGTP, was synthesized and enzymatically incorporated into DNA through primer extension and PCR amplification. The straightforward amplification of super(th)dG-labeled DNA by natural polymerases and simple addition of super(th)dGTP to the standard PCR mix was demonstrated. The present results will facilitate the broad application of long fluorescent DNA.
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ISSN:2046-2069
2046-2069
DOI:10.1039/c4ra05678g