MiR-140 Targets lncRNA DNAJC3-AS1 to Suppress Cell Proliferation in Acute Myeloid Leukemia

• Published in: Mediterranean journal of hematology and infectious diseases Vol. 14; no. 1; p. e2022005
• Main Authors: Li, Hong, Bi, Kehong, Feng, Saran, Wang, Yan, Zhu, Chuansheng
• Format: Journal Article
• Language: English
• Published: Italy Università Cattolica del Sacro Cuore 2022; Mattioli1885
• Subjects: Original; miR-140; DNAJC3-AS1; Proliferation; Acute myeloid leukemia
• ISSN: 2035-3006; 2035-3006
• DOI: 10.4084/MJHID.2022.005

MiR-140 and DNAJC3-AS1 have been demonstrated to play critical roles in cancer biology, while their participation in acute myeloid leukemia (AML) is unclear. This study aimed to explore the role of miR-140 and DNAJC3-AS1 in AML. The expression of DNAJC3-AS1 and miR-140 were detected by RT-qPCR. Then, the role of DNAJC3-AS1 and miR-140 in regulating each other was explored by overexpression assay. Next, the direct interaction between DNAJC3-AS1 and miR-140 was analyzed using an RNA pull-down assay. Next, the subcellular location of DNAJC3-AS1 was explored using cellular, subcellular fractionation assay. Finally, cell proliferation analysis was evaluated with BrdU assay. Increased expression levels of DNAJC3-AS1 and decreased expression levels of miR-140 were observed in AML patients. DNAJC3-AS1 was detected in nuclear and cytoplasm samples and direct interaction between DNAJC3-AS1 and miR-140 was observed. Reduced expression levels of DNAJC3-AS1 were observed after overexpression of miR-140 in AML cells. DNAJC3-AS1 increased cell proliferation and inhibited the role of miR-140 in suppressing cell proliferation. In conclusion, miR-140 may target DNAJC3-AS1 to suppress cell proliferation in AML.