Botryosphaeria spp. as Producers of Laccase When Cultivated on Vegetable Oils as Sole Carbon Source: Optimizing Laccase Production by Botryosphaeria rhodina MAMB-05 on Soybean Oil

• Published in: Orbital : The Electronic Journal of Chemistry Vol. 10; no. 7; pp. 568 - 577
• Main Authors: Sacchetto, Joao P, Filho, Arnaldo F. da Silva, Rezende, Maria I, Scarminio, Ieda, Ayub, Marco A.Z, Giese, Ellen C, Dekker, Robert F.H, Barbosa-Dekker, Aneli M
• Format: Journal Article
• Language: English
• Published: Universidade Federal de Mato Grosso do Sul 01.10.2018
• Subjects: Analysis; botryosphaeria rhodina mamb-05; Glycerol; laccase; Nutritional requirements; Oxidases; Phosphates; response surface methodology; soybean oil, glycerol
• ISSN: 1984-6428; 1984-6428
• DOI: 10.17807/orbital.v10i7.1206

Eight vegetable oils and glycerol were examined as sole carbon source to screen nine isolates of Botryosphaeria spp. Among these, B. rhodina MAMB-05 produced highest laccase titres on all carbon sources evaluated followed by B. rhodina MMLR isolated (from orange, Citrus sp.). The influence of inorganic nitrogen (N[H.sub.4]Cl, NaN[O.sub.3], [(N[H.sub.4]).sub.2]S[O.sub.4], and N[H.sub.4]N[O.sub.3]), organic nitrogen (yeast extract (YE), urea, peptone, and corn steep liquor) sources, inorganic phosphate (K[H.sub.2]P[O.sub.4], [Na.sub.2]HP[O.sub.4], Na[H.sub.2]P[O.sub.4], [K.sub.2]HP[O.sub.4]), as well as increasing concentrations of copper (from zero to 100 [micro]g m[L.sup.-1]), as nutritional requirements for enhancing laccase production by B. rhodina MAMB-05 were also evaluated. Soybean oil was the only carbon source in a central composite factorialdesign ([2.sup.3]) to optimize laccase production with the selected variables NaN[O.sub.3], K[H.sub.2]P[O.sub.4] and copper (CuS[O.sub.4] x 5[H.sub.2]O), using the response surface methodology. The optimal conditions obtained were NaN[O.sub.3] (100 g [L.sup.-1]), K[H.sub.2]P[O.sub.4] (125 g [L.sup.-1]) and 50 [micro]g m[L.sup.-1] of CuS[O.sub.4] x 5[H.sub.2]O. The specific laccase activity obtained (2.95 U [mg.sup.-1]) in the validation experiments using the optimal condition for laccase production by B. rhodina MAMB-05 was in agreement with the predicted value (3.07 U [mg.sup.-1]), and demonstrated the fitness of the proposed model. Keywords: Botryosphaeria rhodina MAMB-05; laccase; soybean oil, glycerol; response surface methodology