Statistical Optimization for Improved Production of Cyclosporin A in Solid-State Fermentation

• Published in: Journal of microbiology and biotechnology Vol. 19; no. 11; pp. 1385 - 1392
• Main Authors: Survase, Shrikant A., University of Mumbai, Mumbai, India, Annapure, Uday S., University of Mumbai, Mumbai, India, Singhal, Rekha S., University of Mumbai, Mumbai, India
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.11.2009; 한국미생물·생명공학회
• Subjects: Ammonium Sulfate - chemistry; Ammonium Sulfate - metabolism; Biological and medical sciences; Biotechnology; Coconut Oil; coconut oil cake; Culture Media - chemistry; Culture Media - metabolism; cyclosporin A; Cyclosporine - metabolism; Dietary Fiber - metabolism; FERMENTACION EN ESTADO SOLIDO; Fermentation; FERMENTATION A L'ETAT SOLIDE; Fundamental and applied biological sciences. Psychology; Glycerol - chemistry; Glycerol - metabolism; Hypocreales - growth & development; Hypocreales - metabolism; Industrial Microbiology - methods; Iron Compounds - chemistry; Iron Compounds - metabolism; Leucine - chemistry; Leucine - metabolism; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Models, Biological; Models, Statistical; Plant Oils - chemistry; Plant Oils - metabolism; SOLID STATE FERMENTATION; Time Factors; Tolypocladium inflatum; Valine - chemistry; Valine - metabolism; wheat bran flour; 생물학; Statistical method; Cereal bran; Tolypocladium inflatum; wheat bran flour; Wheat flour; Solid-state fermentation; Production; cyclosporin A; Solid substrate fermentation; Ciclosporin; Optimization; coconut oil cake
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.0901.0035

This work evaluates the effect of different amino acids on production of Cyclosporin (CyA) production in solid-state fermentation that was previously optimized for different fermentation parameters by one factor at-a-time for the maximum production of CyA by Tolypocladium inflatum MTCC 557. Based on the Plackett-Burman design, glycerol, ammonium sulfate, FeCl₃, and inoculum size were selected for further optimization by response surface methodology (RSM). After identifying effective nutrients, RSM was used to develop mathematical model equations, study responses, and establish the optimum concentrations of the key nutrients for higher CyA production. It was observed that supplementation of medium containing (% w/w) glycerol, 1.53; ammonium sulfate, 0.95; FeCl₃, 0.18; and inoculum size 6.4 ml/5g yielded a maximum of 7,106 mg/kg as compared with 6,480 mg CyA/kg substrate using one factor at-a-time. In the second step, the effect of amino acids on the production of CyA was studied. Addition of L-Valine and L-leucine in combination after 20 h of fermentation resulted in maximum production of 8,166 mg/kg.