Immunodominant Mycobacterium tuberculosis Protein Rv1507A Elicits Th1 Response and Modulates Host Macrophage Effector Functions

• Published in: Frontiers in immunology Vol. 11; p. 1199
• Main Authors: Arora, Simran Kaur, Alam, Anwar, Naqvi, Nilofer, Ahmad, Javeed, Sheikh, Javaid Ahmad, Rahman, Syed Asad, Hasnain, Seyed Ehtesham, Ehtesham, Nasreen Zafar
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 21.07.2020
• Subjects: Animals; Antibodies, Bacterial - immunology; Antigens, Bacterial - immunology; CD4+/CD8+ T cells; central memory; effector memory; Humans; Immunodominant Epitopes; Immunology; Macrophages - immunology; Mice; Mycobacterium smegmatis knock-in; Mycobacterium tuberculosis - immunology; TB subunit vaccine; Th1 Cells - immunology; Tuberculosis - immunology; Tuberculosis Vaccines - immunology; CD4+/CD8+ T cells; TB subunit vaccine; Mycobacterium smegmatis knock-in; effector memory; central memory
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2020.01199

( ) persists as latent infection in nearly a quarter of the global population and remains the leading cause of death among infectious diseases. While BCG is the only vaccine for TB, its inability to provide complete protection makes it imperative to engineer BCG such that it expresses immunodominant antigens that can enhance its protective potential. comparative genomic analysis of Mycobacterium species identified Rv1507A as a "signature protein" found exclusively in . (cell lines) and experiments carried out in mice, using purified recombinant Rv1507A revealed it to be a pro-inflammatory molecule, eliciting significantly high levels of IL-6, TNF-α, and IL-12. There was increased expression of activation markers CD69, CD80, CD86, antigen presentation molecules (MHC I/MHCII), and associated Th1 type of immune response. Rv1507A knocked-in also induced significantly higher pro-inflammatory Th1 response and higher survivability under stress conditions, both (macrophage RAW264.7 cells) and (mice). Sera derived from human TB patients showed significantly enhanced B-cell response against Rv1507A. The ability of Rv1507A to induce immuno-modulatory effect, B cell response, and significant memory response, renders it a putative vaccine candidate that demands further exploration.