Campylobacter concisus Genomospecies 2 Is Better Adapted to the Human Gastrointestinal Tract as Compared with Campylobacter concisus Genomospecies 1

• Published in: Frontiers in physiology Vol. 8; p. 543
• Main Authors: Wang, Yiming, Liu, Fang, Zhang, Xiang, Chung, Heung Kit Leslie, Riordan, Stephen M, Grimm, Michael C, Zhang, Shu, Ma, Rena, Lee, Seul A, Zhang, Li
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 03.08.2017
• Subjects: 23S rRNA; Campylobacter concisus; Crohn's disease; genomospecies; inflammatory bowel disease; Physiology; genomospecies; Crohn's disease; 23S rRNA; Campylobacter concisus; inflammatory bowel disease
• ISSN: 1664-042X; 1664-042X
• DOI: 10.3389/fphys.2017.00543

was previously shown to be associated with inflammatory bowel disease including Crohn's disease (CD) and ulcerative colitis (UC). has two genomospecies (GS). This study systematically examined the colonization of GS1 and GS2 in the human gastrointestinal tract. GS1 and GS2 specific polymorphisms in 23S rRNA gene were identified by comparison of the 23S rRNA genes of 49 strains. Two newly designed PCR methods, based on the polymorphisms of 23S rRNA gene, were developed and validated. These PCR methods were used to detect and quantify GS1 and GS2 in 56 oral and enteric samples collected from the gastrointestinal tract of patients with IBD and healthy controls. Meta-analysis of the composition of the isolated GS1 and GS2 strains in previous studies was also conducted. The quantitative PCR methods revealed that there was more GS2 than GS1 in samples collected from the upper and lower gastrointestinal tract of both patients with IBD and healthy controls, showing that GS2 is better adapted to the human gastrointestinal tract. Analysis of GS1 and GS2 composition of isolated strains in previous studies showed similar findings except that in healthy individuals a significantly lower GS2 than GS1 strains were isolated from fecal samples, suggesting a potential difference in the strains or the enteric environment between patients with gastrointestinal diseases and healthy controls. This study provides novel information regarding the adaptation of different genomospecies of in the human gastrointestinal tract.