Inhibition of Mayaro virus replication by prostaglandin A1 and B2 in Vero cells

• Published in: Brazilian journal of medical and biological research Vol. 31; no. 9; pp. 1119 - 1123
• Main Authors: Ishimaru, D, Marcicano, F G, Rebello, M A
• Format: Journal Article
• Language: English; Portuguese
• Published: Brazil Associação Brasileira de Divulgação Científica 01.09.1998
• Subjects: Alphavirus - drug effects; Alphavirus - growth & development; Alphavirus - physiology; Alphavirus Infections - drug therapy; Animals; BIOLOGY; Cercopithecus aethiops; Glycoproteins - biosynthesis; Mayaro virus; MEDICINE, RESEARCH & EXPERIMENTAL; Methionine - analysis; prostaglandins; Prostaglandins A - metabolism; Prostaglandins A - pharmacology; Prostaglandins A - therapeutic use; Prostaglandins B - metabolism; Prostaglandins B - pharmacology; Prostaglandins B - therapeutic use; Protein C - biosynthesis; protein synthesis; Vero cells; Vero Cells - virology; Virus Replication - drug effects; prostaglandins; protein synthesis; Mayaro virus; Vero cells
• ISSN: 0100-879X; 1414-431X; 1414-431X; 0100-879X
• DOI: 10.1590/S0100-879X1998000900003

The effect of prostaglandins (PGA1 and PGB2) on the replication of Mayaro virus was studied in Vero cells. PGA1 and PGB2 antiviral activity was found to be dose-dependent. However, while 10 micrograms/ml PGB2 inhibited virus yield by 60%, at the same dose PGA1 suppressed virus replication by more than 90%. SDS-PAGE analysis of [35S]-methionine-labelled proteins showed that PGA1 did not alter cellular protein synthesis. In infected cells, PGA1 slightly inhibited the synthesis of protein C, while drastically inhibiting the synthesis of glycoproteins E1 and E2.