Identification by HPLC-PAD-MS and quantification by HPLC-PAD of phenylethanoid glycosides of five Phlomis species

• Published in: Phytochemical analysis Vol. 16; no. 1; pp. 1 - 6
• Main Authors: Kirmizibekmez, H, Montoro, P, Piacente, S, Pizza, C, Donmez, A, Calis, I
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 2005
• Subjects: alyssonoside; chemical structure; Chromatography, High Pressure Liquid - methods; ESI/MS; forsythoside; glycosides; Glycosides - chemistry; HPLC-MS; HPLC-PAD; isoverbascoside; Lamiaceae; leucosceptoside; martynoside; mass spectrometry; medicinal plants; Molecular Structure; phenylethanoid glycoside; phenylethanoid glycosides; Phlomis - chemistry; Phlomis bruguieri; Phlomis kurdica; Phlomis leucophractha; Phlomis nissolii; Phlomis russeliana; Photochemistry; phytochemicals; Plant Components, Aerial - chemistry; plant extracts; qualitative analysis; quantitative analysis; reversed-phase high performance liquid chromatography; samioside; Spectrometry, Mass, Electrospray Ionization - methods; verbascoside; Turkey (country)
• ISSN: 0958-0344; 1099-1565
• DOI: 10.1002/pca.802

The qualitative and quantitative determination of phenylethanoid glycosides in methanolic extracts of five species of the genus Phlomis (Lamiaceae) has been investigated using a new reversed-phase HPLC method combined with photodiode-array detection and electrospray/MS analysis. Forsythoside B, verbascoside, samioside, alyssonoside, isoverbascoside, leucosceptosides A and B and martynoside were detected. The quantification of the above constituents was performed using echinacoside as internal standard. Knowledge of the content of phenylethanoid glycosides contributes to the chemotaxonomy of the genus.