Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus

The sensitivity and specificity of an enzyme-linked immunosorbent assay (ELISA) for the detection of circulating antigens from toxic components of Tityus serrulatus scorpion venom was determined in patients stung by T. serrulatus before antivenom administration. Thirty-seven patients were classified...

Full description

Saved in:
Bibliographic Details
Published inRevista do Instituto de Medicina Tropical de São Paulo Vol. 37; no. 1; pp. 71 - 74
Main Authors de Rezende, N A, Dias, M B, Campolina, D, Chavéz-Olortegui, C, Amaral, C F
Format Journal Article
LanguageEnglish
Published Brazil Instituto de Medicina Tropical 01.02.1995
Subjects
Adolescent
Adult
Animals
Antigens - blood
Child
Child, Preschool
Enzyme-Linked Immunosorbent Assay - standards
Humans
Infant
Scorpion Venoms - immunology
Sensitivity and Specificity
Spider Bites - blood
Spider Bites - diagnosis
Spider Bites - immunology
TROPICAL MEDICINE
Immunodiagnosis
Scorpion envenoming
Online AccessGet full text

Cover

More Information
Summary:The sensitivity and specificity of an enzyme-linked immunosorbent assay (ELISA) for the detection of circulating antigens from toxic components of Tityus serrulatus scorpion venom was determined in patients stung by T. serrulatus before antivenom administration. Thirty-seven patients were classified as mild cases and 19 as moderate or severe cases. The control absorbance in the venom assay was provided by serum samples from 100 individuals of same socioeconomic group and geographical area who had never been stung by scorpions or treated with horse antisera. The negative cutoff value (mean + 2 SD) corresponded to a venom concentration of 4.8 ng/ml. Three out of the 100 normal sera were positive, resulting in a specificity of 97%. The sensitivity of the ELISA when all cases of scorpion sting were included was 39.3%. When mild cases were excluded, the sensitivity increased to 94.7%. This study showed that this ELISA can be used for the detection of circulating venom toxic antigens in patients with systemic manifestations following. T. serrulatus sting but cannot be used for clinical studies in mild cases of envenoming since the test does not discriminate mild cases from control patients.
ISSN:0036-4665
1678-9946
0036-4665
DOI:10.1590/s0036-46651995000100011