Protoxylem: the deposition of a network containing glycine-rich cell wall proteins starts in the cell corners in close association with the pectins of the middle lamella

• Published in: Planta Vol. 216; no. 5; pp. 854 - 864
• Main Author: Ryser, Ulrich
• Format: Journal Article
• Language: English
• Published: Berlin Springer-Verlag 01.03.2003; Springer; Springer Nature B.V
• Subjects: Antibodies; Biological and medical sciences; Cell differentiation; Cell physiology; Cell Wall - metabolism; Cell walls; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Glucans; Glycine max - metabolism; Glycine max - ultrastructure; Hypocotyl - metabolism; Hypocotyl - ultrastructure; Hypocotyls; Microscopy, Electron; Monoclonal antibodies; Parenchyma; Pectins - metabolism; Plant cells; Plant physiology and development; Plant Proteins - metabolism; Plant Structures - metabolism; Plant Structures - ultrastructure; Plants; Polysaccharides; Polysaccharides - metabolism; Receptors, Cytoplasmic and Nuclear - metabolism; Soybeans; Xylans; Xylem; Xylem; Immunocytochemistry; Cell corner; Pectin; Cell differentiation; Glycine; Glycine max; Cell wall; Protein; Hypocotyl; Leguminosae; Protoxylem; Dicotyledones; Angiospermae; Glycine-rich cell wall protein; Spermatophyta; Localization; Polysaccharide; Thickening
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-002-0938-7

Antibodies were used to localise polysaccharide and protein networks in the protoxylem of etiolated soybean (Glycine max L.) hypocotyls. The deposition of glycine-rich proteins (GRPs) starts in the cell corners between protoxylem elements and xylem parenchyma cells. Finally, the GRPs form a network between two mature protoxylem elements. The network also interconnects the ring- and spiral-shaped secondary wall thickenings, as well as the thickenings with the middle lamellae of living xylem parenchyma cells. In addition to the GRP network, a polysaccharide network composed mainly of pectins is involved in the attachment of the secondary wall thickenings to the middle lamellae of xylem parenchyma cells.