Diagnosis and follow-up of Whipple's disease by Amplification of the 16S rRNA gene of Tropheryma whippelii

Amplification of the 16S rRNA gene of Tropheryma whippelii was performed in eight patients with Whipple's disease and 34 control patients to confirm a diagnosis of Whipple's disease and to monitor the course of disease. Polymerase chain reaction (PCR) tests were positive before treatment i...

Full description

Saved in:
Bibliographic Details
Published inEuropean journal of clinical microbiology & infectious diseases Vol. 18; no. 1; pp. 62 - 65
Main Authors PRON, B, POYART, C, HAGEGE, H, BERCHE, P, ABACHIN, E, FEST, T, BELANGER, C, BONNET, C, CAPELLE, P, BRETAGNE, J.-F, FABIANEK, A, GIRARD, L
Format Journal Article
LanguageEnglish
Published Berlin Springer 01.01.1999
Springer Nature B.V
Subjects
Actinobacteria - genetics
Actinobacteria - isolation & purification
Adult
Anti-Bacterial Agents - therapeutic use
Bacterial diseases
Bacterial diseases of the digestive system and abdomen
Biological and medical sciences
Bone marrow
DNA, Bacterial - analysis
Female
Gene Amplification
Human bacterial diseases
Humans
Infectious diseases
Lymph nodes
Male
Medical sciences
Middle Aged
Polymerase Chain Reaction
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
Whipple Disease - diagnosis
Whipple Disease - drug therapy
Whipple Disease - microbiology
Human
Method
Infection
Polymerase chain reaction
Gene amplification
Gene
Intestinal malabsorption
Ribosomal RNA
Bacteriosis
16S-RNA
Digestive diseases
Intestinal disease
Evolution
Diagnosis
Molecular biology
Whipple disease
Online AccessGet full text

Cover

More Information
Summary:Amplification of the 16S rRNA gene of Tropheryma whippelii was performed in eight patients with Whipple's disease and 34 control patients to confirm a diagnosis of Whipple's disease and to monitor the course of disease. Polymerase chain reaction (PCR) tests were positive before treatment in 13 of 15 tissue samples from Whipple's disease patients (gut 8/8; lymph nodes 2/2; bone marrow 1/2; peripheral blood 2/3), in contrast to none of 54 tissue samples from controls. PCR tests converted to negative within 4-6 months in six of the Whipple's disease patients undergoing therapy. These results show that PCR is a reliable and useful tool for diagnosis of Whipple's disease and for monitoring bacterial elimination during antibiotic therapy.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0934-9723
1435-4373
DOI:10.1007/s100960050228