Microextraction in Packed Syringe (MEPS) Utilizing Methylcyanopropyl-Silarylene as Coating Polymer for Extraction of Drugs in Biological Samples

• Published in: Journal of liquid chromatography & related technologies Vol. 29; no. 17; pp. 2537 - 2544
• Main Authors: Abdel-Rehim, Mohamed, Dahlgren, Marie, Blomberg, Lars, Claude, Saturnin, Tabacchi, Raphael
• Format: Journal Article
• Language: English
• Published: Colchester Taylor & Francis Group 01.10.2006; Taylor & Francis
• Subjects: Analysis; Biological and medical sciences; Coating polymer; General pharmacology; Medical sciences; Methylcyanopropyl-silarylene; Microextraction in packed syringe (MEPS); Pharmacology. Drug treatments; Trace analysis; Validation; Biological fluid; Chemical analysis; Coupled method; Coating polymer; Microextraction in packed syringe (MEPS); HPLC chromatography; Ropivacaine; Blood; Chemical enrichment; Microassay; Blood plasma; Local anesthetic; Solid phase microextraction; Methylcyanopropyl-silarylene; Gas chromatography; Sample preparation; Mass spectrometry MS/MS; Syringe; Organic amide; Mass spectrometry
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1080/10826070600915023

Microextraction in packed syringe (MEPS) is a new technique for miniaturised solid-phase extraction that can be connected online to GC or LC. In this work, a liquid polymer was used as coating polymer on a filter in a 10 µL and 250 µL syringe to handle small sample volumes (≤10 µL plasma). Ropivacaine in plasma samples was used as model substance. The validation of the methodology showed that the accuracy values of quality control samples (QC) were in the range of 103%-114% for GC-MS, and 98-101% for LC-MS-MS. The precisions, given as relative standard deviation (R.S.D.) were in the range 1.9 to11% for Inter- and intra-day precisions. The standard curves were obtained within the concentration ranges 5-2,000 nM in human plasma samples. The regression correlation coefficients (R 2 ) for plasma samples were 0.99 for all runs using GC-MS and LC-MS-MS.