Laccase-catalyzed oxidation and intramolecular cyclization of dopamine: A new method for selective determination of dopamine with laccase/carbon nanotube-based electrochemical biosensors

• Published in: Electrochimica acta Vol. 52; no. 12; pp. 4144 - 4152
• Main Authors: Xiang, Ling, Lin, Yuqing, Yu, Ping, Su, Lei, Mao, Lanqun
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 10.03.2007; Elsevier
• Subjects: Biological and medical sciences; Biosensors; Biotechnology; Carbon nanotubes; Dopamine; Electrochemical biosensors; Fundamental and applied biological sciences. Psychology; Laccase; Methods. Procedures. Technologies; Various methods and equipments; Carbon nanotubes; Dopamine; Electrochemical biosensors; Laccase; Catalytic reaction; Enzyme; Selectivity; Determination; Enzyme electrode; Electrochemical detector; Ion selective electrode; Cyclization; Biosensor; Oxidation; Oxidoreductases; Electrochemical reaction
• ISSN: 0013-4686; 1873-3859
• DOI: 10.1016/j.electacta.2006.11.040

This study demonstrates a new electrochemical method for the selective determination of dopamine (DA) with the coexistence of ascorbic acid (AA) and 3,4-dihydroxyphenylacetic acid (DOPAC) with laccase/multi-walled carbon nanotube (MWNT)-based biosensors prepared by cross-linking laccase into MWNT layer confined onto glassy carbon electrodes. The method described here is essentially based on the chemical reaction properties of DA including oxidation, intramolecular cyclization and disproportionation reactions to finally give 5,6-dihydroxyindoline quinone and on the uses of the two-electron and two-proton reduction of the formed 5,6-dihydroxyindoline quinone to constitute a method for the selective determination of DA at a negative potential that is totally separated from those for the redox processes of AA and DOPAC. Instead of the ECE reactions of DA with the first oxidation of DA being driven electrochemically, laccase is used here as the biocatalyst to drive the first oxidation of DA into its quinone form and thus initialize the sequential reactions of DA finally into 5,6-dihydroxyindoline quinone. In addition, laccase also catalyzes the oxidation of AA and DOPAC into electroinactive species with the concomitant reduction of O 2. As a consequence, a combinational exploitation of the chemical properties inherent in DA and the multifunctional catalytic properties of laccase as well as the excellent electrochemical properties of carbon nanotubes substantially enables the prepared laccase/MWNT-based biosensors to be well competent for the selective determination of DA with the coexistence of physiological levels of AA and DOPAC. This demonstration offers a new method for the selective determination of DA, which could be potentially employed for the determination of DA in biological systems.