Solid-Phase Extraction Followed by Purification with Tri-n-Butylphosphate as a Simple and Useful Rat Plasma Clean-up Method for HPLC-UV Determination of Zonampanel Monohydrate (YM872), a Highly Water-Soluble α-Amino-3-hydroxy-5-methylisoxazole-4-propionic Acid Receptor Antagonist

• Published in: Journal of liquid chromatography & related technologies Vol. 31; no. 7; pp. 1072 - 1080
• Main Authors: Miyashita, Aiji, Hashimoto, Tadashi, Aoki, Toshiko, Soeishi, Yoshiaki, Kamimura, Hidetaka, Noguchi, Kiyoshi
• Format: Journal Article
• Language: English
• Published: Colchester Taylor & Francis Group 29.02.2008; Taylor & Francis
• Subjects: AMPA Receptor antagonist; Analysis; Biological and medical sciences; General pharmacology; HPLC; Medical sciences; Pharmacology. Drug treatments; Plasma; UV detection; YM872; Zonampanel monohydrate; Nitro compound; Imidazole derivatives; Solid phase extraction; Biological fluid; Plasma; AMPA Receptor antagonist; Chemical analysis; Intravenous administration; Rat; Nitrogen heterocycle; HPLC chromatography; Glutamate receptor; Blood; Zonampanel monohydrate; Chemical enrichment; Blood plasma; Sample preparation; Carboxylic acid; Bicyclic compound; Aromatic compound; Antagonist; HPLC; Quantitative analysis; Biological receptor; Trace analysis; UV detection; Rodentia; Zonampanel; Water soluble compound; Vertebrata; AMPA receptor; Mammalia; Ultraviolet detector; YM872; Animal; Pharmacokinetics
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1080/10826070801925607

A high-performance liquid chromatographic method with ultraviolet detection was developed for the determination of zonampanel monohydrate (YM872), a novel α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor antagonist, in rat plasma. YM872 was extracted via solid-phase extraction. To remove blank plasma derived endogenous peaks, the extract was further purified by washing with tri-n-butyl phosphate and injected onto reversed-phase HPLC with ultraviolet detection at 333 nm. The precision and accuracy were less than 4.99% and within±7.89%, respectively. The limit of quantification was 10 ng/mL when 1 mL of plasma was used. The usefulness of this method was demonstrated by monitoring plasma concentrations after intravenous administration of YM872 to rats.