Fluorescence Assay for Detecting Four Organophosphorus Pesticides Using Fluorescently Labeled Aptamer

• Published in: Sensors (Basel, Switzerland) Vol. 22; no. 15; p. 5712
• Main Authors: Li, He, Huang, Xue, Huang, Jingcheng, Bai, Mengyuan, Hu, Mengjiao, Guo, Yemin, Sun, Xia
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 30.07.2022; MDPI
• Subjects: Agricultural production; aptamer; Binding sites; Chromatography; Electron transfer; Fluorescence; fluorescence assay; guanine; organophosphorus pesticide; Pesticides; tetramethylrhodamine; China
• ISSN: 1424-8220; 1424-8220
• DOI: 10.3390/s22155712

In this work, we reported a rapid and sensitive fluorescence assay in homogenous solution for detecting organophosphorus pesticides by using tetramethylrhodamine (TAMRA)-labeled aptamer and its complementary DNA (cDNA) with extended guanine (G) bases. The hybridization of cDNA and aptamer drew TAMRA close to repeated G bases, then the fluorescence of TAMRA was quenched by G bases due to the photoinduced electron transfer (PET). Upon introducing the pesticide target, the aptamer bound to pesticide instead of cDNA because of the competition between pesticide and cDNA. Thus, the TAMRA departed from G bases, resulting in fluorescence recovery of TAMRA. Under optimal conditions, the limits of detection for phorate, profenofos, isocarbophos, and omethoate were 0.333, 0.167, 0.267, and 0.333 µg/L, respectively. The method was also used in the analysis of profenofos in vegetables. Our fluorescence design was simple, rapid, and highly sensitive, which provided a means for monitoring the safety of agricultural products.