Optimization using a Factorial Design for the Separation of Trandolapril and Verapamil by Capillary Electrophoresis

• Published in: Journal of liquid chromatography & related technologies Vol. 30; no. 20; pp. 2975 - 2988
• Main Authors: Capella-Peiró, María-Elisa, Font-Rubert, Manolo, Àlvarez-Rodríguez, Lluís, Esteve-Romero, Josep, Durgbanshi, Abhilasha, Bose, Devasish
• Format: Journal Article
• Language: English
• Published: Colchester Taylor & Francis Group 01.10.2007; Taylor & Francis
• Subjects: Analysis; Biological and medical sciences; Capillary electrophoresis; Cardiac drugs; Factorial design; General pharmacology; Medical sciences; Pharmacology. Drug treatments; Trandolapril; Verapamil; Chemical analysis; Peptides; Nitrogen heterocycle; Calcium antagonist; Trandolapril; Optimization; Peptidyl-dipeptidase A; Aralkylamine; Bicyclic compound; Tablet; Quantitative analysis; Enzyme; Capillary electrophoresis; Enzyme inhibitor; Oral form; Antiarrhythmic agent; Cardiac drugs; Peptidases; Ultraviolet detector; Experimental design; Dipeptides; Dosage form; Hydrolases; Peptidyl-dipeptidases; Verapamil; Hard capsule; Factorial design; ACE inhibitor; Chemometrics
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1080/10826070701629465

A capillary zone electrophoresis method is developed to achieve the separation and determination of two cardiac drugs, trandolapril and verapamil. Optimisation of the method is based on the use of a factorial design, which is applied in order to obtain more information in the factorial space and to minimise the number of experiments needed to obtain the optimum values for the pH and concentration of electrolyte buffer, the voltage applied and the effects of certain modifiers. Following these studies, the conditions selected for use were running buffer of 10 mM phosphate at pH 7.0, and a voltage of 15 kV. Under these conditions, calibration curves were constructed with good linearity (R>0.999) and suitable LODs. The intra- and inter-day repeatabilities were also evaluated with RSD below 4% for trandolapril and verapamil. The proposed method was applied to the determination of verapamil and trandolapril in pharmaceutical formulations and the recoveries obtained were in agreement with the stated contents for all preparations tested.