Optimization of extraction condition for major iridoid components in fruit of corni (Cornus officinalis) by UPLC-PDA using response surface methodology

• Published in: Food science and biotechnology Vol. 21; no. 4; pp. 1023 - 1029
• Main Authors: Lee, A.Y., Korea Institute of Oriental Medicine, Daejeon, Republic of Korea, Kim, H.S., Korea Institute of Oriental Medicine, Daejeon, Republic of Korea, Jo, J.E., Korea Institute of Oriental Medicine, Daejeon, Republic of Korea, Kang, B.K., Korea Institute of Oriental Medicine, Daejeon, Republic of Korea, Moon, B.C., Korea Institute of Oriental Medicine, Daejeon, Republic of Korea, Chun, J.M., Korea Institute of Oriental Medicine, Daejeon, Republic of Korea, Ji, Y.U., Korea Institute of Oriental Medicine, Daejeon, Republic of Korea, Kim, H.K., Korea Institute of Oriental Medicine, Daejeon, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society of Food Science and Technology 01.08.2012; 한국식품과학회
• Subjects: Chemistry; Chemistry and Materials Science; Cornus; Ethanol; EXTRACCION; EXTRACTION; Food Science; fruit of Cornus officinalis; Fruits; Liquid chromatography; Nutrition; optimization; Regression analysis; Research Article; response surface methodology; Temperature effects; ultra performance liquid chromatography-photodiode array detector; 식품과학; fruit of; response surface methodology; optimization; ultra performance liquid chromatography-photodiode array detector
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-012-0133-y

The fruit of corni (Cornus officinalis Sieb.) was analyzed by ultra-performance liquid chromatography with a photodiode array detector (UPLC-PDA) and method validation for 3 major components which are morroniside, loganin, and cornuside. The optimal extraction condition for sum of 3 components was carried out response surface methodology (RSM). Experimental factors were designated by RSM and a central composite design (CCD). The suitability of the experimental model was verified by analysis of variance (ANOVA), and the regression coefficient was calculated as 0.6994. The maximum predicted value for the sum of the yields of the 3 major components was 1.555% with an extraction temperature of 70℃ and an extraction time of 38 min in 64% ethanol. Under these conditions, the actual value was detected to 1.527% (±0.034).