A WRKY transcription factor confers broad-spectrum resistance to biotic stresses and yield stability in rice

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 122; no. 10; p. e2411164122
• Main Authors: Liu, Daoming, He, Jun, Li, Qi, Zhang, Xiao, Wang, Yongsheng, Sun, Quanguang, Wang, Wenhui, Zhang, Menglong, Wang, Yunlong, Xu, Haosen, Fang, Liang, Jiang, Ling, Liu, Shijia, Chen, Liangming, Tian, Yunlu, Liu, Xi, Wang, Ruyi, Zhang, Zhengguang, Chern, Mawsheng, Dong, Xiaoou, Wang, Haiyang, Liu, Yuqiang, Ronald, Pamela C., Wan, Jianmin
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 11.03.2025
• Subjects: Ammonia; Animals; Biological Sciences; Biosynthesis; Cultivars; Disease Resistance - genetics; Gene Expression Regulation, Plant; Gene silencing; Insects; Lignin - biosynthesis; Oryza - genetics; Oryza - growth & development; Oryza - metabolism; Pathogens; Pests; Phenylalanine; Phenylalanine Ammonia-Lyase - genetics; Phenylalanine Ammonia-Lyase - metabolism; Plant Diseases - genetics; Plant Diseases - parasitology; Plant Proteins - genetics; Plant Proteins - metabolism; Rice; Sheaths; Stress, Physiological - genetics; Transcription Factors - genetics; Transcription Factors - metabolism; insect; broad-spectrum resistance; disease; rice; yield
• ISSN: 0027-8424; 1091-6490; 1091-6490
• DOI: 10.1073/pnas.2411164122

Plants are subject to attack by diverse pests and pathogens. Few genes conferring broad-spectrum resistance to both insects and pathogens have been identified. Because of the growth–defense tradeoff, it is often challenging to balance biotic stress resistance and yield for crops. Here, we report that OsWRKY36 suppresses the resistance to insects and pathogens via transcriptional repression of Phenylalanine Ammonia Lyases ( PALs ), a key enzyme in phenylpropanoid pathway in rice. Knocking out OsWRKY36 causes elevated lignin biosynthesis and increased sclerenchyma thickness of leaf sheath, leading to enhanced resistance to multiple pests and pathogens. Additionally, loss of OsWRKY36 also derepresses the transcription of Ideal Plant Architecture 1 ( IPA1 ) and MONOCULM2 ( MOC2 ), resulting in increased spikelet number per panicle and tiller number. These findings provide mechanistic insights into biotic stress tolerance in rice and offer a promising strategy to breed rice cultivars with broad-spectrum resistance to insects and pathogens while maintaining stable yield.