Purification, elicitor activity, and cell wall localization of a glycoprotein from Phytophthora parasitica var. nicotianae, a fungal pathogen of tobacco

ABSTRACT A glycoprotein of 34 kDa (GP 34) was solubilized at acidic pH from the mycelium of Phytophthora parasitica var. nicotianae and was purified by ion exchange and gel permeation chromatography. Whole tobacco plants treated with GP 34 through their roots showed an enhanced lipoxygenase activity...

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Published inPhytopathology Vol. 87; no. 9; pp. 899 - 909
Main Authors SEJALON-DELMAS, N, VILLALBA MATEOS, F, BOTTIN, A, RICKAUER, M, DARGENT, R, ESQUERRE-TUGAYE, M. T
Format Journal Article
LanguageEnglish
Published St. Paul, MN American Phytopathological Society 01.09.1997
Subjects
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Pathology, epidemiology, host-fungus relationships. Damages, economic importance
Phytopathology. Animal pests. Plant and forest protection
Immunohistochemistry
Plant pathogen
Phycomycetes
Phytophthora nicotianae
Stimulant plant
Host agent relation
Glycoproteins
Elicitor
Nicotiana tabacum
Cell wall
Fungi
Dicotyledones
Angiospermae
Defense mechanism
Spermatophyta
Solanaceae
Localization
Thallophyta
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Summary:ABSTRACT A glycoprotein of 34 kDa (GP 34) was solubilized at acidic pH from the mycelium of Phytophthora parasitica var. nicotianae and was purified by ion exchange and gel permeation chromatography. Whole tobacco plants treated with GP 34 through their roots showed an enhanced lipoxygenase activity as well as hydroxyproline-rich glycoprotein accumulation, indicating that this molecule had elicitor properties. An antiserum raised against the pure glycoprotein allowed localization of GP 34 by immunogold-labeling on the cell surface of the mycelium when the fungus was grown in vitro. In the wall-less zoospores, GP 34 was limited to the flagellum surface. It was then abundantly synthesized at the onset of encystment. During infection of tobacco plants, labeling was very faint at early stages of colonization, particularly in the susceptible host cultivar. It appeared earlier in the resistant host cultivar and was restricted to the living fungus, declining with mycelium cell death.
Bibliography:ObjectType-Article-1
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ISSN:0031-949X
1943-7684
DOI:10.1094/PHYTO.1997.87.9.899