Influence of reduction in the elution times on HPLC glycohaemoglobin results

• Published in: Clinical biochemistry Vol. 42; no. 15; pp. 1582 - 1584
• Main Authors: Leon-Justel, Antonio, Santotoribio, Jose D., Dominguez-Pascual, Inmaculada, Delgado, Ana L., Macias, Carlos, Herrera, Maria T., Leal, Alfonso, Mangas, Miguel A., Acosta, Domingo, Guerrero, J.M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2009
• Subjects: Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Diabetes; Elution times; Fetal Hemoglobin - analysis; Glycated Hemoglobin A - analysis; Glycohaemoglobin; Hemoglobin, Sickle - analysis; HPLC; Humans; Time Factors; PBS; L-A1C; Elution times; Hb; Diabetes; HbA1c; S-A1c; HPLC; DCCT; Glycohaemoglobin

To compare HPLC methods with short and long elution times for HbA1c measurement in blood. Comparison between G7-Tosoh (1.2 min); Bio-Rad-Variant-II-Turbo (1.3 min) and Arkray-HA-8160 (2.9 min). Passing–Bablok regression equations were: Y = 0.17 + 0.96 X; Y = − 0.39 + 1.01 X; Y = − 0.40 + 1.0 X and t...