Influence of reduction in the elution times on HPLC glycohaemoglobin results

To compare HPLC methods with short and long elution times for HbA1c measurement in blood. Comparison between G7-Tosoh (1.2 min); Bio-Rad-Variant-II-Turbo (1.3 min) and Arkray-HA-8160 (2.9 min). Passing–Bablok regression equations were: Y = 0.17 + 0.96 X; Y = − 0.39 + 1.01 X; Y = − 0.40 + 1.0 X and t...

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Published inClinical biochemistry Vol. 42; no. 15; pp. 1582 - 1584
Main Authors Leon-Justel, Antonio, Santotoribio, Jose D., Dominguez-Pascual, Inmaculada, Delgado, Ana L., Macias, Carlos, Herrera, Maria T., Leal, Alfonso, Mangas, Miguel A., Acosta, Domingo, Guerrero, J.M.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.10.2009
Subjects
Chromatography, High Pressure Liquid - instrumentation
Chromatography, High Pressure Liquid - methods
Diabetes
Elution times
Fetal Hemoglobin - analysis
Glycated Hemoglobin A - analysis
Glycohaemoglobin
Hemoglobin, Sickle - analysis
HPLC
Humans
Time Factors
PBS
L-A1C
Elution times
Hb
Diabetes
HbA1c
S-A1c
HPLC
DCCT
Glycohaemoglobin
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Summary:To compare HPLC methods with short and long elution times for HbA1c measurement in blood. Comparison between G7-Tosoh (1.2 min); Bio-Rad-Variant-II-Turbo (1.3 min) and Arkray-HA-8160 (2.9 min). Passing–Bablok regression equations were: Y = 0.17 + 0.96 X; Y = − 0.39 + 1.01 X; Y = − 0.40 + 1.0 X and the means of the differences using Bland–Altman Plot were 0.02; − 0.34; 0.32 for HA-8160/G7-Tosoh, HA-8160/Variant-II-Turbo and G7-Tosoh/Variant-II-Turbo, respectively. Faster elution methods had no problems on reproducibility of results obtained by slower elution methods.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2009.07.003