Ribosomal DNA sequences of Glugea anomala, G. stephani, G. americanus and Spraguea lophii (Microsporidia) : phylogenetic reconstruction

• Published in: Diseases of aquatic organisms Vol. 40; no. 2; pp. 125 - 129
• Main Authors: POMPORT-CASTILLON, C, DE JONCKHEERE, J. F, ROMESTAND, B
• Format: Journal Article
• Language: English
• Published: Oldendorf Inter-Research 14.03.2000
• Subjects: Animals; Biological and medical sciences; Brackish; DNA, Protozoan - chemistry; DNA, Ribosomal - chemistry; Freshwater; Fundamental and applied biological sciences. Psychology; Glugea americanus; Glugea anomala; Glugea atherinae; Glugea stephani; Marine; Microsporida - genetics; Phylogeny; Polymerase Chain Reaction - veterinary; Protozoa; rRNA; Spraguea lophii; Systematics. Geographical distribution. Morphology. Cytology; Protozoa; Host parasite relation; Nucleotide sequence; Taxonomy; Interspecific comparison; Ribosomal DNA; Chemotaxonomy; Parasite; Zoopathogen; Phylogeny; Subunit; Cnidosporidia; Vertebrata; Glugea anomala; Pisces; Glugea stephani
• ISSN: 0177-5103; 1616-1580
• DOI: 10.3354/dao040125

The microsporidian species Glugea anomala, G. stephani, G. americanus and Spraguea lophii were compared by using sequence data derived from their small subunit rDNA genes which were amplified by polymerase chain reaction and directly sequenced. These sequence data and published data of G. atherinae were analyzed and were used to infer a phylogenetic tree. The 5 microsporidian fish parasites appeared to be closely related. The higher sequence similarities demonstrated among G. anomala, G. stephani and G. atherinae suggest that these 3 parasites are in fact only 1 species of Glugea. Moreover, the higher sequence similarities between S. lophii and G. americanus support the transfer of the latter Glugea species into the genus Spraguea.