Multiple DNA markers for evaluation of resistance against Potato virus Y, Potato virus S and Potato leafroll virus

Molecular markers within or close to genes of interest play essential roles in marker-assisted selection. PCR-based markers have been developed for numerous traits in different plant species including several genes conferring resistance to viruses in potato. In the present work, rapid and reliable a...

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Bibliographic Details
Published inCzech Journal of Genetics and Plant Breeding Vol. 54; no. 1; pp. 30 - 33
Main Authors NADERPOUR, Masoud, SADEGHI, Leila
Format Journal Article
LanguageEnglish
Czech
Slovak
Published Prague Czech Academy of Agricultural Sciences (CAAS) 01.01.2018
Czech Academy of Agricultural Sciences
Subjects
Amplification
Biomarkers
Deoxyribonucleic acid
Disease resistance
DNA
Genes
Genotyping
Marker-assisted selection
multiplex genotyping
Plant breeding
Polerovirus
Polymerase chain reaction
Potatoes
solanum tuberosum
virus resistance
Viruses
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Summary:Molecular markers within or close to genes of interest play essential roles in marker-assisted selection. PCR-based markers have been developed for numerous traits in different plant species including several genes conferring resistance to viruses in potato. In the present work, rapid and reliable approaches were developed for the simultaneous detection of Ryadg and Ry-fsto, Ns, and PLRV.1 genes conferring resistance to Potato virus Y, Potato virus S and Potato leafroll virus, respectively, on the basis of previously published and newly modified markers. The sequence characterized amplified region (SCAR) markers for Ryadg, Ns and PLRV1 and the newly modified cleaved amplified polymorphic sequences (CAPS) marker for Ry-fsto were amplified in one PCR reaction which could simply characterize Ryadg and PLRV.1 resistance. Additional digestion of amplicons with EcoRV and MfeI for genotyping the Ry-fsto and Ns resistance genes, respectively, was needed. The effectiveness of genotyping in triplex and tetraplex PCRs was tested on 35 potato varieties used for potato seed production and breeding programs.
ISSN:1212-1975
1805-9325
DOI:10.17221/180/2016-CJGPB