Cavitation mechanism in cyanobacterial growth inhibition by ultrasonic irradiation

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 33; no. 3; pp. 151 - 156
• Main Authors: Hao, Hongwei, Wu, Minsheng, Chen, Yifang, Tang, Jiaowen, Wu, Qingyu
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.02.2004
• Subjects: Cavitation intensity; Gas vesicle; Growth inhibition; Spirulina; Ultrasonic irradiation; Spirulina; Gas vesicle; Growth inhibition; Cavitation intensity; Ultrasonic irradiation
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2003.09.003

To prevent cyanobacterial bloom in eutrophic water by ultrasonic method, ultrasonic irradiations with different parameters were tested to inhibit Spirulina platensis from growth. The experimental result based on cyanobacterial growth, chlorophyll a and photosynthetic activity showed that, the ultrasonic irradiation inhibited cyanobacterial proliferation effectively, furthermore the inhibition effectiveness increased in the order: 200 kHz>1.7 MHz>20 kHz and became saturated with the increased power. The inhibition mechanism can be mainly attributed to the mechanical damage to the cell structures caused by ultrasonic cavitation, which was confirmed by light microscopy and differential interference microscopy. The optimal frequency of 200 kHz in cavition and sonochemistry was also most effective in cyanobacterial growth inhibition. The higher frequency of 1.7 MHz is weaker than 20 kHz in cavitation, but has more effective inhibition because it is nearer to the resonance frequency of gas vesicle. The inhibition saturation with ultrasonic power was due to the ultrasonic attenuation induced by the acoustic shielding of bubbles enclosing the radiate surface of transducer.