RNA extraction method from fruit tissue high in water and sugar

RNA isolation from ripe fruit can be complicated by high concentrations of sugar and water. These sugars interfere with RNA extraction often resulting in low RNA quality and quantities, and high water concentrations dilute the RNA, making isolation difficult. We report a simple but novel method by w...

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Bibliographic Details
Published inHortScience Vol. 41; no. 5; pp. 1292 - 1294
Main Authors Davis, A.R, Levi, A, Kim, S, King, S.R, Hernandez, A
Format Journal Article
LanguageEnglish
Published Alexandria, VA American Society for Horticultural Science 01.08.2006
Subjects
Agronomy. Soil science and plant productions
Biological and medical sciences
cantaloupes
Chemical constitution
Citrullus lanatus
Cucumis melo subsp. melo var. cantalupensis
Economic plant physiology
extraction
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
genetic techniques and protocols
Genetics and breeding of economic plants
RNA
Solanum lycopersicum var. lycopersicum
soluble solids
sugar content
tomatoes
water content
watermelons
Vegetables
Horticulture
RNA
Citrullus lanatus
rapid
Biochemistry
tomato
RNA isolation
sugar removal
Dicotyledones
Angiospermae
Lycopersicon esculentum
Water content
Carbohydrate
liquid removal
Solanaceae
cucurbit
Nucleic acid
Sugar
Plant tissue
Composition
Fruit
Extraction process
Biochemical compound
Desiccation
Cucurbitaceae
Vegetable crop
Fruit crop
Isolation
Spermatophyta
Cucumis melo
Molecular biology
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Summary:RNA isolation from ripe fruit can be complicated by high concentrations of sugar and water. These sugars interfere with RNA extraction often resulting in low RNA quality and quantities, and high water concentrations dilute the RNA, making isolation difficult. We report a simple but novel method by which the majority of the excess sugar and water in mature fruit of tomato (Lycopersicon esculentum Mill.), watermelon Citrullus lanatus (Thunb.) Matsum. & Nakai, and muskmelon (Cucumis melo L.) can be easily removed from tissue before RNA extraction. This method produced quality RNA in a shorter time than the currently accepted method for fruit tissue RNA isolation and does not require liquid nitrogen or a freeze dryer.
Bibliography:http://hdl.handle.net/10113/18460
ISSN:0018-5345
2327-9834
DOI:10.21273/hortsci.41.5.1292