Enantioseparation of Chlorpheniramine by High Speed Countercurrent Chromatography Using Carboxymethyl-β-cyclodextrin as Chiral Selector

• Published in: Journal of liquid chromatography & related technologies Vol. 28; no. 19; pp. 3057 - 3063
• Main Authors: Yuan, L. M., Liu, J. C., Yan, Z. H., Ai, P., Meng, X., Xu, Z. G.
• Format: Journal Article
• Language: English
• Published: Colchester Taylor & Francis Group 01.11.2005; Taylor & Francis
• Subjects: Analytical chemistry; Biological and medical sciences; Carboxymethyl-β-cyclodextrin; Chemistry; Chlorpheniramine; Chromatographic methods and physical methods associated with chromatography; Enantioseparation; Exact sciences and technology; General pharmacology; High speed countercurrent chromatography; Medical sciences; Other chromatographic methods; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Countercurrent chromatography; High speed countercurrent chromatography; Separation; Pharmaceutical technology; Carboxymethyl-β-cyclodextrin; Oligosaccharide; Preparative chromatography; Antihistaminic; Chiral selector; Cyclodextrin; Chlorpheniramine; Enantioseparation; Optical resolution; Enantiomer; Antagonist; H1 Histamine receptor; Chlorphenamine; Inclusion compound
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1080/10826070500295138

High speed countercurrent chromatography was used for enantioseparation of chlorpheniramine. The separation was performed with a two-phase system composed of ethyl acetate∶methanol∶water (10∶1∶9) within 2 hours in a tail-to-head elution mode. The lower phase contained 20 mmol/L of carboxymethly-β-cyclodextrin as a chiral selector. The enantiomers separated were identified by chiral HPLC, which confirmed that this method was very useful for the chiral preparative separation.