HPLC-MS/MS analysis of 9 artificial sweeteners in imported foods

• Published in: Food science and biotechnology Vol. 22; no. Suppl 1; pp. 233 - 240
• Main Authors: Lim, Ho-Soo, Park, Sung-Kwan, Kwak, In-Shin, Kim, Hyung-Il, Sung, Jun-Hyun, Jang, Su-Jin, Byun, Mi-Youn, Kim, So-Hee
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society of Food Science and Technology 01.02.2013; 한국식품과학회
• Subjects: Centrifugation; Chemistry; Chemistry and Materials Science; Food Science; Nutrition; Research Article; 식품과학; imported food; HPLC-MS; artificial sweetener
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-013-0072-2

An analytical method for the simultaneous determination of 9 artificial sweeteners (acesulfame-K, cyclamate, sodium saccharin, aspartame, alitame, neotame, sucralose, dulcin, and neohesperidine dihydrochalcone) in imported foods by HPLC-MS/MS was developed. Samples were extracted with buffer solution (pH 4.5). The supernatant was analyzed by HPLC-MS/MS after centrifugation and filtration. The sample was separated on a thermo hypersil BPS C 18 (250×3 mm, 5-μm) column, and detected by MS/MS with selective reaction monitoring (SRM) mode. The correlation coefficients ( r 2 ) of the calibration curve were >0.99. The recoveries were 90.0–107.5% with good coefficients of variation of 1.8–8.6%. The limits of detection and limits of quantification were between 0.001 and 0.375 mg/mL and between 0.003 and 1.125 mg/mL, respectively. The proposed method has been successfully applied to the determination of the 9 sweeteners in various foods.