Degradation potential and diversity of oil-degrading bacteria isolated from the sediments of the Jiaozhou Bay, China

• Published in: Acta oceanologica Sinica Vol. 38; no. 6; pp. 54 - 64
• Main Authors: Gao, Wei, Gao, Xiangxing, Mi, Tiezhu, Han, Bin, Zhang, Yiran, Li, Xinzi, Yin, Xiaofei, Sun, Chengjun, Li, Qian, Cui, Zhisong, Luan, Xiao, Yu, Zhigang, Zheng, Li
• Format: Journal Article
• Language: English
• Published: Beijing The Chinese Society of Oceanography 01.06.2019; Springer Nature B.V; Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China%Marine Ecology Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China; Key Laboratory of Marine Chemical Theory and Technology, Ministry of Education, Qingdao 266100, China; Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China%Marine Ecology Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China%Key Laboratory of Marine Chemical Theory and Technology, Ministry of Education, Qingdao 266100, China; Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China; Marine Ecology Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China%Marine Ecology Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China; National Deep Sea Center, Qingdao 266237, China%College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China; College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
• Subjects: Alkanes; Analysis; Aromatic hydrocarbons; Bacteria; Biodegradation; Biodiversity; Bioremediation; Carbon sources; Climatology; Consortia; Crude oil; Degradation; DNA sequencing; Earth and Environmental Science; Earth Sciences; Ecology; Ecosystems; Energy resources; Energy sources; Engineering Fluid Dynamics; Environmental Chemistry; Gene sequencing; Gravimetric analysis; Marine & Freshwater Sciences; Microbiological strains; Oceanography; Oil; Oil pollution; Petroleum pipelines; Polycyclic aromatic hydrocarbons; rRNA 16S; Sediment; Sediment samplers; Sediment samples; Sediments; Sequence analysis; Shorelines; Strains (organisms); Submarine pipelines; dominant bacteria; biodegradation; oil spill; microbial consortium
• ISSN: 0253-505X; 1869-1099
• DOI: 10.1007/s13131-019-1353-2

A great deal of oil contaminated the shoreline by the Qingdao oil pipeline explosion in 2013. The four oil-degrading consortia were enriched from sediment samples with crude oil as sole carbon and energy sources. The biodiversity and community analysis showed that the Luteibacter , Parvibaculum and a genus belonging to Alcanivoracaceae were found predominant bacteria in the four consortia, which belonged to Proteobacteria . Nine strains exhibiting distinct 16S rRNA gene sequences were isolated from the consortia. These strains were identified to eight genera based on 16S rRNA gene sequences. Five of the nine strains degraded more than 30% of the crude oil in two weeks by gravimetric method. From the analysis of GC-MS, most of the isolated strains tended to degrade n-alkanes rather than PAHs. Five strains showed high degrading ability of the total n-alkanes. Only Strain D2 showed great PAHs degrading ability and the degrading rates ranged from 34.9% to 77.5%. The sequencing analysis of the oil-degrading consortia confirmed that the genus of Alcanivorax was one of the dominant bacteria in Consortia A and E and Strain E4 might be one of the dominant bacteria. The strains obtained in this study demonstrated the potential for oil bioremediation in oil-contaminated beach ecosystems.