A Fluorescence Spot Test for Salicylate Determination

• Published in: Analytical letters Vol. 40; no. 3; pp. 573 - 583
• Main Authors: Linares, E. M., Palermo, L. T., Moreira, A. B., Sotomayor, M. D. P. T, Kubota, L. T.
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Taylor & Francis Group 01.02.2007; Taylor & Francis
• Subjects: Analytical chemistry; ASA; biological samples; Chemistry; Exact sciences and technology; Fluorescence spectroscopy; pharmaceuticals; salicylic acid; Spectrometric and optical methods; Fluorescence spectroscopy; Biological compound; Salicylic acid; Spot test; Detection limit; Standard deviation; Filter paper; pharmaceuticals; Fluorescence spectrometry; biological samples; Optical fiber; ASA
• ISSN: 0003-2719; 1532-236X
• DOI: 10.1080/00032710600966010

A procedure based on a spot test for salicylate is presented. The method is simple, rugged and is based on the salycilate native fluorescence measurement on the surface of a filter paper by using optical fibers for light conduction. The fluorescence was linear at concentration range of 0.01-0.4 mmol l −1 . The relative standard deviation of 5% (n=10) was observed. Detection and quantification limits were 4 and 14 µmol l −1 , respectively. The results obtained in pharmaceutical salicylate determinations showed that practically no statistical difference existed with the pharmacopeical method (95% confidence level). The recovery studies in biological samples spiked with salicylic acid at overdose concentration levels showed that the matrix sample did not interfere in the method.