Estrogen receptor-α signaling in tanycytes lies at the crossroads of fertility and metabolism

• Published in: Metabolism, clinical and experimental Vol. 158; p. 155976
• Main Authors: Fernandois, Daniela, Rusidzé, Mariam, Mueller-Fielitz, Helge, Sauve, Florent, Deligia, Eleonora, Silva, Mauro S.B., Evrard, Florence, Franco-García, Aurelio, Mazur, Daniele, Martinez-Corral, Ines, Jouy, Nathalie, Rasika, S., Maurage, Claude-Alain, Giacobini, Paolo, Nogueiras, Ruben, Dehouck, Benedicte, Schwaninger, Markus, Lenfant, Francoise, Prevot, Vincent
• Format: Journal Article Web Resource
• Language: English
• Published: United States Elsevier Inc 01.09.2024; Elsevier; Elsevier BV
• Subjects: Animals; Endocrinologie, métabolisme & nutrition; Endocrinology, metabolism & nutrition; Ependymoglial Cells - metabolism; Estrogen Receptor alpha - genetics; Estrogen Receptor alpha - metabolism; Estrogens; Estrous Cycle - metabolism; Estrous Cycle - physiology; Female; Female metabolism; Fertility - physiology; Gonadotropin-Releasing Hormone - metabolism; Human health sciences; Hypothalamus; Hypothalamus - metabolism; LH pulsatility; Life Sciences; Luteinizing Hormone - metabolism; Mice; Mice, Inbred C57BL; Neurons - metabolism; Neuropeptide Y - metabolism; Ovariectomy; Reproduction; Sciences de la santé humaine; Signal Transduction - physiology; Tanycytes; Reproduction; Hypothalamus; LH pulsatility; Estrogens; Female metabolism; Tanycytes
• ISSN: 0026-0495; 1532-8600; 1532-8600
• DOI: 10.1016/j.metabol.2024.155976

Estrogen secretion by the ovaries regulates the hypothalamic-pituitary-gonadal axis during the reproductive cycle, influencing gonadotropin-releasing hormone (GnRH) and luteinizing hormone (LH) secretion, and also plays a role in regulating metabolism. Here, we establish that hypothalamic tanycytes—specialized glia lining the floor and walls of the third ventricle—integrate estrogenic feedback signals from the gonads and couple reproduction with metabolism by relaying this information to orexigenic neuropeptide Y (NPY) neurons. Using mouse models, including mice floxed for Esr1 (encoding estrogen receptor alpha, ERα) and those with Cre-dependent expression of designer receptors exclusively activated by designer drugs (DREADDs), along with viral-mediated, pharmacological and indirect calorimetric approaches, we evaluated the role of tanycytes and tanycytic estrogen signaling in pulsatile LH secretion, cFos expression in NPY neurons, estrous cyclicity, body-weight changes and metabolic parameters in adult females. In ovariectomized mice, chemogenetic activation of tanycytes significantly reduced LH pulsatile release, mimicking the effects of direct NPY neuron activation. In intact mice, tanycytes were crucial for the estrogen-mediated control of GnRH/LH release, with tanycytic ERα activation suppressing fasting-induced NPY neuron activation. Selective knockout of Esr1 in tanycytes altered estrous cyclicity and fertility in female mice and affected estrogen's ability to inhibit refeeding in fasting mice. The absence of ERα signaling in tanycytes increased Npy transcripts and body weight in intact mice and prevented the estrogen-mediated decrease in food intake as well as increase in energy expenditure and fatty acid oxidation in ovariectomized mice. Our findings underscore the pivotal role of tanycytes in the neuroendocrine coupling of reproduction and metabolism, with potential implications for its age-related deregulation after menopause. Our investigation reveals that tanycytes, specialized glial cells in the brain, are key interpreters of estrogen signals for orexigenic NPY neurons in the hypothalamus. Disrupting tanycytic estrogen receptors not only alters fertility in female mice but also impairs the ability of estrogens to suppress appetite. This work thus sheds light on the critical role played by tanycytes in bridging the hormonal regulation of cyclic reproductive function and appetite/feeding behavior. This understanding may have potential implications for age-related metabolic deregulation after menopause. •Hypothalamic tanycytes provide estradiol-weighed information to orexigenic NPY neurons in females•ERα in tanycytes links estrogen signaling to appetite and fertility in cycling females•ERα expression in female tanycytes is required for estrogens to exert its anorexigenic effect•Tanycytic ERα signaling is required for fasting-induced cFos expression in NPY neurons and refeeding in females.