The Establishment of a New En Bloc Combined Liver-Small Bowel Transplantation Model in Rats

• Published in: Transplantation proceedings Vol. 39; no. 1; pp. 278 - 280
• Main Authors: Zhao, Y, Lu, S, Cheng, F, Shen, L.-Z, Hua, Y.-B, Wu, W.-X
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 2007
• Subjects: Animals; Graft Survival; Intestine, Small - transplantation; Liver Transplantation; Male; Mesenteric Artery, Superior - surgery; Models, Animal; Portal Vein - surgery; Rats; Rats, Wistar; Surgery; Transplantation, Isogeneic - methods; Vena Cava, Inferior - surgery
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2006.10.010

Abstract In order to facilitate preclinical research, we established a new combined liver-small bowel transplantation rat model. Male inbred Wistar rats were chosen as donors and recipients. An en bloc liver-small bowel graft was harvested. During the donor operation, the inferior vena cava in the chest was removed to be used as an interpositional venous graft to anastomose to the portal vein. In the recipient operation the portal veins of donor and recipient were quickly anastomosed using a cuff technique instead of the traditional suture method. Rearterialization was achieved by anastomosing the superior mesenteric artery of graft to the right renal artery of the recipient. The recipient small bowel was resected and intestinal continuity restored simultaneously by two end-to-end anastomoses. The postoperative 5-day survival rate was 77.5% (31/40) and 60-day survival rate, 72.5% (29/40). Recipient rats that tolerated the operation remained healthy. Liver and renal function was normal. The liver and intestinal grafts showed normal histological architecture in all rats surviving for 2 months postoperatively. Our results demonstrated that the present model is feasible, allowing preclinical experimental research on combined liver-small bowel transplantation.