Caffeine: The Allelochemical Responsible for the Plant Growth Inhibitory Activity of Vietnamese Tea (Camellia sinensis L. Kuntze)

• Published in: Agronomy (Basel) Vol. 9; no. 7; p. 396
• Main Authors: PHAM, Van Thi Thanh, ISMAIL, Tamer, MISHYNA, Maryia, APPIAH, Kwame Sarpong, OIKAWA, Yosei, FUJII, Yoshiharu
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 18.07.2019
• Subjects: Allelochemicals; Allelopathy; Caffeine; Camellia sinensis; Green tea; Herbicides; High performance liquid chromatography; Leachates; Leaves; Liquid chromatography; Phytotoxicity; Plant extracts; Plant growth; Plant species; Seedlings; Seeds; Soils; specific activity tea; Tea; total activity; Weed control; Weeds; Vietnam
• ISSN: 2073-4395; 2073-4395
• DOI: 10.3390/agronomy9070396

The present study aimed to examine the phytotoxic potential of seven Vietnamese tea samples based on the specific and total activity of caffeine and tea extracts on test plants. The sandwich method results indicated that the inhibitory effect of tea samples on the radicle and hypocotyl growth of lettuce seedlings was dependent on the concentration and type of tea samples, and also the presence of agar soluble allelochemicals. Among the seven tea samples, the leachates from Vinatea-green tea showed the highest inhibition on the radicle growth of lettuce seedlings with 50% suppression at 0.12 mg dry leaves/mL of agar. Caffeine concentration in tea samples analyzed by high-performance liquid chromatography (HPLC) varied from 20.7 to 38.2 µg/mL of dry leaves. The specific activity (EC50 value) of pure caffeine was 75 µg/mL, and the highest total activity of caffeine estimated in Vinatea-green tea was 0.51 [no unit]. Caffeine from green and oolong tea may be considered as one of the contributors to the inhibitory activity of the crude extract. Moreover, the phytotoxicity of pure caffeine and aqueous tea extracts was highly selective on the growth of different plant species. The concentration of caffeine detected from tea farm soil ranged from 0.137 to 0.145 µg/g soil. The results indicated that caffeine might be considered as a promising allelochemical from Vietnamese tea and can be a good candidate for weed management.