Acute toxicity effects of perfluorooctane sulfonate on sperm vitality, kinematics and fertilization success in zebrafish

• Published in: Chinese journal of oceanology and limnology Vol. 35; no. 4; pp. 723 - 728
• Main Author: 夏继刚 牛翠娟
• Format: Journal Article
• Language: English
• Published: Heidelberg Science Press 01.07.2017; Springer Nature B.V
• Subjects: Activation analysis; Acute toxicity; Angular velocity; Aquatic environment; Biological fertilization; Biology; Contaminants; Danio rerio; Displacement; Earth and Environmental Science; Earth Sciences; Exposure; Fertilization; Fish; Fish reproduction; Freshwater fishes; Kinematics; Media; Natural environment; Oceanography; Perfluorooctane sulfonic acid; PFOS; Pollution; reproduction; Sperm; Spermatozoa; Sulfonates; Toxicity; Velocity; Viability; Zebrafish; 全氟辛烷; 受精; 急性毒性效应; 斑马鱼; 磺酸; 精子活力; 运动学; sperm kinematics; fertilization success; perfluorooctane sulfonate (PFOS); acute toxicity; sperm viability
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-017-6086-5

Perfluorooctane sulfonate （PFOS） has emerged as one of the most concerning contaminants in recent years. This study aimed to investigate the acute toxicity effect of PFOS on sperm viability, kinematics and fertilization success in zebrafish （Danio rerio）. Sperm were activated in aqueous media containing a range of PFOS concentrations （0, 0.09, 0.9 and 9 mg/L）. Viabilities and kinematics of the sperm exposed to different PFOS treatments were assessed via computer-assisted sperm analysis （CASA） at 20, 40, 60, and 80 s after activation. PFOS exposure decreased the percentage of motile sperm, the curvilinear velocity （VCL）, and the mean angular displacement （MAD） of spermatozoa, but showed no influence on the straight- line velocity （VSL） or the angular path velocity （VAP）. Furthermore, a significant decrease in fertilization success was observed in spermatozoa that were exposed to 0.9 mg/L PFOS or more. These findings indicate that PFOS pollution in natural aquatic environment may be a potential threaten to successful reproduction of fish.