Short Communication: Expression of APOBEC3G and Interferon Gamma in Pleural Fluid Mononuclear Cells from HIV/TB Dual Infected Subjects

• Published in: AIDS research and human retroviruses Vol. 31; no. 7; p. 692
• Main Authors: Toossi, Zahra, Meng, Qinglai, Aung, Htin, Liu, Shigou, Mayanja-Kizza, Harriet, Hirsch, Christina S
• Format: Journal Article
• Language: English
• Published: United States 01.07.2015
• Subjects: Adult; APOBEC-3G Deaminase; Body Fluids - cytology; Body Fluids - virology; Cytidine Deaminase - biosynthesis; Gene Expression Profiling; HIV Infections - complications; HIV Infections - immunology; HIV Infections - virology; HIV-1 - isolation & purification; Humans; Interferon-gamma - biosynthesis; Leukocytes, Mononuclear - immunology; Male; Pleural Effusion; Tuberculosis, Pulmonary - complications; Tuberculosis, Pulmonary - immunology; Viral Load
• ISSN: 1931-8405
• DOI: 10.1089/AID.2014.0297

Sites of HIV/TB coinfection are characterized by increased HIV-1 replication and a TH1 profile. However, expression of HIV-1 restriction factors, such as APOBEC3G (A3G) in situ, is unknown. Using an RT-profiler focused on genes related to HIV-1 expansion, we examined pleural fluid mononuclear cells (PFMCs) from patients with HIV/TB coinfection in comparison to HIV-uninfected patients with TB disease. Significant expression of interferon (IFN)-γ and restriction factors A3G and A3F and TRIM5α in PFMCs was found. Genes correlating significantly with the expression of IFN-γ included A3G and A3F. However, pleural fluid HIV-1 viral load and HIV-1 gag/pol mRNA in PFMCs did not correlate with A3G activity.