Hematopoietic cell transplantation using plasma and DMSO without HES, with non-programmed freezing by immersion in a methanol bath : results in 213 cases

• Published in: Bone marrow transplantation (Basingstoke) Vol. 21; no. 5; pp. 511 - 517
• Main Authors: HERNANDEZ-NAVARRO, F, OJEDA, E, GARCIA-MIGUEL, P, MARTINEZ, A, SASTRE, A, CALERO, F, GOMEZ-PASTRANA, F, MARTINEZ, B, ARRIETA, R, RIOS-RULL, P, GARCIA-BUSTOS, J, QUEVEDO, E, HERNANDEZ, M. P. M, JIMENEZ-YUSTE, V, RODRIGUEZ-LUACES, M, LOPEZ, R. M
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing Group 01.03.1998
• Subjects: Ablation; Adolescent; Adult; Aged; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Autografts; Biological and medical sciences; Blood; Blood Component Removal; Blood Specimen Collection - methods; Bone marrow; Bone Marrow Cells; Bone Marrow Transplantation - methods; Bone marrow, stem cells transplantation. Graft versus host reaction; Cell Survival; Cells (biology); Chemotherapy; Child; Child, Preschool; Cryopreservation; Cryopreservation - methods; Cryoprotective Agents; Dimethyl Sulfoxide; Female; Freezing; Granulocytes; Hematopoietic Stem Cell Transplantation - methods; Hematopoietic Stem Cells; Hemopoiesis; Humans; Hydroxyethyl Starch Derivatives; Infant; Leukocytes (granulocytic); Male; Medical sciences; Methanol; Middle Aged; Neoplasms; Neoplasms - therapy; Osteoprogenitor cells; Patients; Peripheral blood; Pilot Projects; Plasma Substitutes; Platelets; Progenitor cells; Software; Solvents; Stem cell transplantation; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Transplantation; Case study; Human; Autograft; Radiation injury; Digestive diseases; Intestinal disease; Complication; Graft; Male; Colitis; Radiotherapy; Hyperbaric oxygenotherapy
• ISSN: 0268-3369; 1476-5365
• DOI: 10.1038/sj.bmt.1701125

A simplified cryopreservation method for bone marrow (BM) and peripheral blood progenitor cells (PBPC) was utilized in hematopoietic cell transplantation of 213 patients with hematological or solid neoplasms after ablative chemotherapy (187 with peripheral blood progenitor cells and 26 with bone marrow). Cells were cryopreserved, after addition of autologous fresh plasma with DMSO, without HES, by freezing to -80 degrees C in a methanol bath and non-programmed freezer. For the patients autotransplanted with PBPC, the median period necessary for recovery of more than 0.5 x 10(9)/l granulocytes was 11 days (range 6-44), and 15 (8-204) days were required to obtain more than 20 x 10(9)/l platelets. For the patients autotransplanted with BM, the median period necessary to recover >0.5 x 10(9)/l granulocytes was 12 days (range 9-33), and 24 (12-57) days to obtain more than 20 x 10(9)/l platelets. These results support this method as being very effective in achieving high-quality cryopreservation. The procedure, which uses a non-programmed freezer, simplifies and reduces enormously the cost of the technical measures currently in use, enabling its adoption in almost any clinical oncological institution.