Vanillin Improves and Prevents Trinitrobenzene Sulfonic Acid-Induced Colitis in Mice

• Published in: The Journal of pharmacology and experimental therapeutics Vol. 330; no. 2; pp. 370 - 376
• Main Authors: Wu, Shih-Lu, Chen, Jaw-Chyun, Li, Chia-Cheng, Lo, Hsin-Yi, Ho, Tin-Yun, Hsiang, Chien-Yun
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2009; American Society for Pharmacology and Experimental Therapeutics
• Subjects: Animals; Benzaldehydes - therapeutic use; Colitis - chemically induced; Colitis - pathology; Colitis - prevention & control; Female; Mice; Mice, Inbred BALB C; Mice, Transgenic; Trinitrobenzenesulfonic Acid - toxicity
• ISSN: 0022-3565; 1521-0103; 1521-0103
• DOI: 10.1124/jpet.109.152835

Inflammatory bowel disease (IBD) is chronic inflammatory and relapsing disease of the gut. It has been known that activation of nuclear factor-κB (NF-κB) and production of proinflammatory cytokines play important roles in the pathogenesis of IBD. In this study, the effect of vanillin (4-hydroxy-3-methoxybenzaldehyde), a potent nuclear factor-κB (NF-κB) inhibitor, was evaluated in mice with trinitrobenzene sulfonic acid (TNBS)-induced colitis. Oral administration of vanillin improved macroscopic and histological features of TNBS-induced colitis in a dose-dependent manner. Vanillin not only prevented TNBS-induced colitis but also ameliorated the established colitis. By in vivo NF-κB bioluminescence imaging, electrophoretic mobility shift assay, and Western blot, we found that vanillin suppressed in vivo NF-κB activities through the inhibition of p65 translocation, inhibitor of nuclear factor-κB(IκB)-α phosphorylation, and IκB kinase activation. Furthermore, vanillin reduced the expressions of proinflammatory cytokines [interleukin (IL)-1β, IL-6, interferon-γ, and tumor necrosis factor-α] and stimulated the expression of anti-inflammatory cytokine (IL-4) in colonic tissues. In conclusion, this work identified vanillin as an anti-inflammatory compound with the capacity to prevent and ameliorate TNBS-induced colitis. Due to its safety, vanillin could be a potent candidate for the treatment of IBD.