Nelumbo nucifera leaves protect hydrogen peroxide-induced hepatic damage via antioxidant enzymes and HO-1/Nrf2 activation
Naturally occurring phenolic compounds are widely found in plants. Here, the phenolic composition and hepatoprotective effect of the butanolic extract (BE) from Nelumbo nucifera leaves against H 2 O 2 -induced hepatic damage in cultured hepatocytes were investigated. BE showed high total phenol and...
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Published in | Food & function Vol. 6; no. 6; pp. 1911 - 1918 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
England
01.06.2015
|
Subjects | |
Online Access | Get full text |
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Summary: | Naturally occurring phenolic compounds are widely found in plants. Here, the phenolic composition and hepatoprotective effect of the butanolic extract (BE) from
Nelumbo nucifera
leaves against H
2
O
2
-induced hepatic damage in cultured hepatocytes were investigated. BE showed high total phenol and flavonoid contents, and major phenolic compounds are quercetin, catechin, ferulic acid, rutin, and protocatechuic acid by HPLC analysis. BE effectively scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) cation radicals (IC
50
values of 5.21 μg mL
−1
for DPPH and 6.22 μg mL
−1
for ABTS
+
) and showed strong reducing power. Pretreatment of BE prior to 650 μM H
2
O
2
exposure markedly increased cell viability and suppressed H
2
O
2
-induced intracellular reactive oxygen species generation and AAPH-induced cell membrane lipid peroxidation. In addition, BE up-regulated intracellular glutathione levels under normal and oxidative stress conditions. Notably, the hepatoprotective effect of BE was directly correlated with the increased expression of superoxide dismutase-1 (SOD-1) by 0.62-fold, catalase (CAT) by 0.42-fold, and heme oxygenase-1 (HO-1) by 2.4-fold. Pretreatment of BE also increased the nuclear accumulation of Nrf2 by 8.1-fold indicating that increased SOD-1, CAT, and HO-1 expressions are Nrf2-mediated.
Nelumbo nucifera
leaves ameliorated hepatotoxicity
via
antioxidant action. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2042-6496 2042-650X |
DOI: | 10.1039/c5fo00201j |