Cloning, characterization and expression analysis of a microsomal glutathione S-transferase gene from the seagrass Zostera marina

• Published in: Acta oceanologica Sinica Vol. 38; no. 10; pp. 111 - 115
• Main Authors: Yan, Wenjie, Liu, Jiao, Seng, Samphal, Zhou, Bin, Ding, Kuke
• Format: Journal Article
• Language: English
• Published: Beijing The Chinese Society of Oceanography 01.10.2019; Springer Nature B.V; Key Laboratory of Radiological Protection and Nuclear Emergency,Chinese Center for Disease Control and Prevention,Beijing 100088,China; Department of Marine Ecology,College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China%Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China%Faculty of Fisheries & Aquaculture,Royal University of Agriculture,Phnom Penh 12101,Cambodia%National Institute for Radiological Protection,Chinese Center for Disease Control and Prevention,Beijing 100088,China
• Subjects: Climatology; Cloning; Disease control; E coli; Earth and Environmental Science; Earth Sciences; Ecology; Engineering Fluid Dynamics; Environmental Chemistry; Enzymes; Escherichia coli; Gene expression; Glutathione; Glutathione transferase; Marine & Freshwater Sciences; Oceanography; Phylogenetics; Proteins; Recombinants; Sea grasses; Short message service; Stability analysis; Temperature; Temperature changes; Temperature effects; Temperature tolerance; Temperature variations; Thermal stability; Zostera marina; antioxidant enzyme; temperature; glutathione S-transferase (GST); enzyme activity; Zostera marina
• ISSN: 0253-505X; 1869-1099
• DOI: 10.1007/s13131-019-1429-z

The response of glutathione S-transferase (GST) in Zostera marina to temperature variation was analyzed at molecular level by cloning the microsomal GST gene and texting the microsomal GST expression regularity under different temperature. Specific speaking, express Zm GST in Escherichia coli , then purify the recombinant protein and make the thermal stability analysis. Therefore, the experiments were carried out to provide a theoretical basis for the further elaboration to the population degradation mechanisms of Z. marina . In conclusion, the thermostability and the response of Zm GST gene to temperature changes can determine its temperature tolerance range, and affect its resilience in turn.