FTIR spectroscopic analysis of Saccharomyces cerevisiae cell walls: study of an anomalous strain exhibiting a pink-colored cell phenotype

• Published in: FEMS microbiology letters Vol. 197; no. 2; pp. 179 - 186
• Main Authors: Galichet, A, Sockalingum, G.D, Belarbi, A, Manfait, M
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.04.2001; Blackwell; Oxford University Press
• Subjects: alpha-glucosidase; alpha-Glucosidases - genetics; Archaea; b-glucans; Bacteriology; Baking yeast; beta-glucans; Biological and medical sciences; Cell viability; Cell Wall - chemistry; cell wall components; Cell wall structure; Cell walls; Complementation; Fourier transform infrared spectroscopy; FTIR spectroscopy; Fundamental and applied biological sciences. Psychology; genes; Genetic Vectors; Genetics; Glucans; Glucans - analysis; Glucosidase; Infrared spectroscopy; Mannoprotein; Mannoproteins; Membrane Glycoproteins - analysis; Microbiology; mutants; Mutation; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; phenotype; Phenotypes; Saccharomyces cerevisiae; Saccharomyces cerevisiae - chemistry; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - ultrastructure; spectral analysis; Spectroscopy, Fourier Transform Infrared; Thermococcus; Thermococcus - genetics; Thermococcus hydrothermalis; Transformation, Genetic; Yeast; Yeasts; α-Glucosidase; β-Glucan; β-Glucan; α-Glucosidase; Mannoprotein; Cell wall structure; FTIR spectroscopy; Yeast; Archaeobacteria; Cell wall; Fungi; Thermococcales; Gene; Bacteria; Thermococcus; Ascomycetes; Complementation; Cell; Thallophyta; Clone; Evaluation; Enzyme; Color; Thermococcaceae; Strain; Phenotype; Decrease; Glycosidases; Hydrolases; Mutation; O-Glycosidases; Saccharomyces cerevisiae
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.2001.tb10601.x

A new strain, exhibiting an intriguing pink-colored cell phenotype, was obtained after an encoding alpha-glucosidase gene from an archaebacteria Thermococcus hydrothermalis was cloned by functional complementation of a mal11 Saccharomyces cerevisiae mutant TCY70. The possible implications of the alpha-glucosidase on the cell wall were evaluated by infrared spectroscopy and data indicate a 30% decrease in mannoproteins and an increase in beta-glucans. The loss of mannoproteins was confirmed by experiments on cells deprived of peptidomannans. Modifications in the major components of the cell wall did not jeopardize cell viability. Such rapid optical spectroscopic method can be used to screen a wide range of yeast mutants.