Hypoxic Stress Stably Alters Apoptotic Parameters on U937 Cells

: Tumor promonocytic U937 cells cultured under a low O2/high CO2 atmosphere display altered characteristics after restoration of normal atmosphere: increased resistance to apoptosis induced by different treatments; apoptotic morphology; lack of glutathione (GSH) extrusion in apoptosis; lack of prote...

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Bibliographic Details
Published inAnnals of the New York Academy of Sciences Vol. 1010; no. 1; pp. 426 - 429
Main Authors DE NICOLA, M, LIUZZI, F, CERELLA, C, D'ALESSIO, M, BERGAMASCHI, A, MAGRINI, A, GHIBELLI, L
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.12.2003
Subjects
Antioxidants - metabolism
apoptosis
Apoptosis - drug effects
Apoptosis - physiology
Calcium Signaling - physiology
Cell Hypoxia - physiology
glutathione
Glutathione - metabolism
Humans
hypoxia
Puromycin - pharmacology
U937
U937 Cells
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Summary:: Tumor promonocytic U937 cells cultured under a low O2/high CO2 atmosphere display altered characteristics after restoration of normal atmosphere: increased resistance to apoptosis induced by different treatments; apoptotic morphology; lack of glutathione (GSH) extrusion in apoptosis; lack of protection by antioxidants; and lack of Ca2+ mobilization with thapsigargin. These alterations were stably maintained for many months of culture in normal conditions, originating the stable U937‐HX variant. Since the hypoxic treatment did not produce a great selective pressure, the alterations are conceivably the result of stable adaptative response.
Bibliography:istex:91C65768C39AE238F7DEE10B93EBFB0EB6512905
ark:/67375/WNG-T865HCWG-H
ArticleID:NYAS426
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0077-8923
1749-6632
DOI:10.1196/annals.1299.076