Automated Analysis of Hop Bittering Components with High Performance Liquid Chromatography Using Precolumn Switching

An automated method based on on-line sample enrichment by precolumn switching has been developed for the determination of hop bittering components in beer and wort. A combination of two columns was used: a tetramethylolmethanetriacrylate type for trapping and an ODS silica type for final separation....

Full description

Saved in:
Bibliographic Details
Published inAnalytical Sciences Vol. 2; no. 3; pp. 235 - 238
Main Authors ONO, Miyoko, KAKUDO, Youichi, UEDA, Suehiro
Format Journal Article
LanguageEnglish
Published Tokyo The Japan Society for Analytical Chemistry 1986
Japan Society for Analytical Chemistry
Japan Science and Technology Agency
Subjects
Automated HPLC
beer
Beers
Biological and medical sciences
direct injection
Fermented food industries
Food industries
Fundamental and applied biological sciences. Psychology
on-line sample enrichment
precolumn switching
wort
Assay
Hop
Liquid chromatography
Bitter compound
Online AccessGet full text

Cover

More Information
Summary:An automated method based on on-line sample enrichment by precolumn switching has been developed for the determination of hop bittering components in beer and wort. A combination of two columns was used: a tetramethylolmethanetriacrylate type for trapping and an ODS silica type for final separation. Iso-α-acids and α- acids were determined with similar results by the conventional method, based on off-line sample enrichment (liquid- liquid extraction and concentration). The analysis was carried out with good reproducibilities (rsd less than 3%). Precolumn switching is distinguished by injection of beer and wort samples without preteatment, and is eminently suited for automated routine analysis in breweries.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.2.235