Crizotinib loaded polydopamine–polylactide-TPGS nanoparticles in targeted therapy for non-small cell lung cancer

• Published in: Medical oncology (Northwood, London, England) Vol. 40; no. 1; p. 26
• Main Authors: Wang, Han, Wu, Yilan, Lin, Xiaoyan
• Format: Journal Article
• Language: English
• Published: New York Springer US 02.12.2022; Springer Nature B.V
• Subjects: Apoptosis; Carcinoma, Non-Small-Cell Lung - drug therapy; Chemical and Drug Induced Liver Injury; Crizotinib - pharmacology; Hematology; Humans; Internal Medicine; Liver; Lung cancer; Lung Neoplasms - drug therapy; Medicine; Medicine & Public Health; Nanoparticles; Oncology; Original Paper; Pathology; Targeted cancer therapy; Crizotinib; Nanoparticles; Liver toxicity; Non-small cell lung cancer; Apoptosis
• ISSN: 1559-131X; 1357-0560; 1559-131X
• DOI: 10.1007/s12032-022-01893-8

To evaluate the effect and safety of crizotinib loaded polydopamine–polylactide-TPGS nanoparticles (CZT/pD–PT NPs) on non-small cell lung cancer (NSCLC). CZT/pD–PT NPs were synthesized and characterized, and their effects on PC-9 cell viability and apoptosis were determined. In vivo experiment was further performed to evaluate the anti-NSCLC efficacy of CZT/pD–PT NPs. TUNEL assay and Western blot were respectively applied for the determination of cell apoptosis and apoptosis-related protein expression, while liver function-related index expression detection and liver histopathological detection were used to evaluate the hepatotoxicity of CZT/pD–PT NPs. Compared with free CZT, CZT/pD–PT NPs had a sustained-release effect and promoted the cellular uptake of CZT. In addition, CZT/pD–PT NPs significantly inhibited PC-9 cell viability and promoted cell apoptosis both in vitro and in vivo, exhibiting superior cytotoxicity. At the same time, CZT/pD–PT NPs had no significant effect on liver tissue morphology and liver function-related indicators such as ALP, ALT, AST, and DBIL. CZT/pD–PT NPs have excellent anti-NSCLC effect with low hepatotoxicity, which can be served as a novel drug delivery system to improve the efficacy of chemotherapy for NSCLC.