40 years of glyco-polyacrylamide in glycobiology

• Published in: Glycoconjugate journal Vol. 38; no. 1; pp. 89 - 100
• Main Authors: Tuzikov, Alexander, Chinarev, Alexander, Shilova, Nadezhda, Gordeeva, Elena, Galanina, Oxana, Ovchinnikova, Tatyana, Schaefer, Marcel, Bovin, Nicolai
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.02.2021; Springer Nature B.V
• Subjects: Acrylic Resins - chemistry; Biochemistry; Biomedical and Life Sciences; Biotin; Biotin - chemistry; Biotin - metabolism; Entropy; Glycoconjugates - chemical synthesis; Glycoconjugates - chemistry; Glycomics - methods; Life Sciences; Molecular Weight; Orthomyxoviridae - metabolism; Pathology; Polyacrylamide; Polymers; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - metabolism; Reproducibility of Results; Review Article; Fluorescent probes; Glyc-PAA; Glycan-binding proteins; Immobilization; Solid-phase assay; Glycans; Oligosaccharides; PGA; PAA; Glyco-surface; End-biotin; Glycopolymers; Glyco-clusters

Polyacrylamide conjugates of glycans have long been widely used in many research areas of glycobiology, mainly for immobilizing glycans in solid-phase assays and as multivalent inhibitors. Pending biotin tag allows immobilizing Glyc-PAA quantitatively on any surface, and acts as a tracer for detecti...