40 years of glyco-polyacrylamide in glycobiology

• Published in: Glycoconjugate journal Vol. 38; no. 1; pp. 89 - 100
• Main Authors: Tuzikov, Alexander, Chinarev, Alexander, Shilova, Nadezhda, Gordeeva, Elena, Galanina, Oxana, Ovchinnikova, Tatyana, Schaefer, Marcel, Bovin, Nicolai
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.02.2021; Springer Nature B.V
• Subjects: Acrylic Resins - chemistry; Biochemistry; Biomedical and Life Sciences; Biotin; Biotin - chemistry; Biotin - metabolism; Entropy; Glycoconjugates - chemical synthesis; Glycoconjugates - chemistry; Glycomics - methods; Life Sciences; Molecular Weight; Orthomyxoviridae - metabolism; Pathology; Polyacrylamide; Polymers; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - metabolism; Reproducibility of Results; Review Article; Fluorescent probes; Glyc-PAA; Glycan-binding proteins; Immobilization; Solid-phase assay; Glycans; Oligosaccharides; PGA; PAA; Glyco-surface; End-biotin; Glycopolymers; Glyco-clusters
• ISSN: 0282-0080; 1573-4986; 1573-4986
• DOI: 10.1007/s10719-020-09965-5

Polyacrylamide conjugates of glycans have long been widely used in many research areas of glycobiology, mainly for immobilizing glycans in solid-phase assays and as multivalent inhibitors. Pending biotin tag allows immobilizing Glyc-PAA quantitatively on any surface, and acts as a tracer for detection of carbohydrate-binding proteins. However, the scope of already realized capabilities of these probes is immeasurably richer than those listed above. This review is not so much about routine as about less common, but not less significant applications. Also, the data on the glycopolymers themselves, their molecular weight, size and polymer chain flexibility are presented, as well as the methods of synthesis, clusterisation and entropy factor in their interaction with proteins.