Delphinidin diminishes in vitro interferon-γ and interleukin-17 producing cells in patients with psoriatic disease

• Published in: Immunologic research Vol. 70; no. 2; pp. 161 - 173
• Main Authors: Tsiogkas, Sotirios G., Mavropoulos, Αthanasios, Skyvalidas, Dimitrios N., Patrikiou, Eleni, Ntavari, Niki, Daponte, Athina Ioanna, Grammatikopoulou, Maria G., Dardiotis, Efthimios, Roussaki-Schulze, Aggeliki-Victoria, Sakkas, Lazaros I., Zafiriou, Efterpi, Bogdanos, Dimitrios P.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.04.2022; Springer Nature B.V
• Subjects: Allergology; Anthocyanins - therapeutic use; Arthritis; Arthritis, Psoriatic - drug therapy; Biomedical and Life Sciences; Biomedicine; CD4 antigen; CD8 antigen; CD8-Positive T-Lymphocytes; Cell culture; Cytokines; Functional foods & nutraceuticals; Humans; Immunology; Immunomodulation; Inflammation; Interferon-gamma; Interleukin 10; Interleukin 17; Internal Medicine; Ionomycin; Leukocytes, Mononuclear; Lymphocytes; Lymphocytes T; Medicine/Public Health; Original Article; Peripheral blood mononuclear cells; Psoriasis; Psoriatic arthritis; γ-Interferon; IFNγ; Diet supplement; IL-17A; Psoriasis; Immunoregulation; Inflammation; Immunonutrition; PBMCs
• ISSN: 0257-277X; 1559-0755; 1559-0755
• DOI: 10.1007/s12026-021-09251-y

The anthocyanidin delphinidin reduces psoriasiform lesions and inflammatory mediators in human cell culture systems. Its role in psoriatic disease has not yet been investigated. We assessed delphinidin’s in vitro immunomodulatory effect on ex vivo stimulated peripheral blood mononuclear cells (PBMCs) from 50 individuals [26 with psoriasis, 10 with psoriatic arthritis (PsA) and 14 healthy controls (HCs)]. Cells were either left untreated or stimulated with PMA plus ionomycin in the presence or absence of delphinidin. Intracellular production of interferon-γ (IFNγ), interleukin-17A (IL-17A), and interleukin-10 (IL-10) was measured flow cytometrically. Delphinidin dose-dependently reduced IFNγ + T cells from patients and HCs. The mean IFNγ decrease in CD4 + T subpopulations was 42.5 ± 28% for psoriasis patients, 51.8 ± 21.5% for PsA patients and 49 ± 17% for HCs ( p  < 0.001 for all). Similarly, IFNγ reduction in CD8 + T cells was 34 ± 21.6% for psoriasis patients, 47.1 ± 22.8% for PsA and 44.8 ± 14.3% for HCs ( P < 0.001 for all). An inhibitory effect of delphinidin was also noted in IFNγ producing NKs and NKTs from psoriasis individuals. Delphinidin also significantly decreased IL-17 + CD4 + T cells in all tested subjects, with marginal effect on the increase of IL-10-producing T regulatory subsets. In conclusion, delphinidin exerts a profound in vitro anti-inflammatory effect in psoriasis and psoriatic arthritis by inhibiting IFNγ + innate and adaptive cells and T helper (Th) 17 cells. If this effect is also exerted in vivo, delphinidin may be regarded as a nutraceutical with immunosuppressive potential.