Shrimp AHPND Causing Vibrio anguillarum Infection: Quantitative Diagnosis and Identifying Antagonistic Bacteria
Acute hepatopancreatic necrosis disease (AHPND) is one of the most common and serious diseases in shrimp aquaculture. Relevant works have focused on the gut microbiota-disease relationship when serious AHPND occurs. In contrast, little is known about how the gut microbiota responds to pathogen infec...
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Published in | Marine biotechnology (New York, N.Y.) Vol. 23; no. 6; pp. 964 - 975 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.12.2021
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Acute hepatopancreatic necrosis disease (AHPND) is one of the most common and serious diseases in shrimp aquaculture. Relevant works have focused on the gut microbiota-disease relationship when serious AHPND occurs. In contrast, little is known about how the gut microbiota responds to pathogen infection over AHPND progression, whereas this knowledge is fundamental to uncover the etiology of AHPND. Here, we explored the temporal succession of shrimp gut microbiota during
Vibrio anguillarum
(a causal pathogen of AHPND) challenge. The successful infection of
V. anguillarum
was confirmed by linearly increased abundance of the pathogen in the shrimp gut over AHPND progression.
V. anguillarum
infection caused an irreversible disruption in the shrimp gut microbiota, of which infection and hours post infection (hpi) respectively constrained 6.2% and 10.2% of variation in the data. Furthermore, the predicted functional pathways involved in immunity and metabolism significantly decreased, while those facilitating infectious diseases significantly enriched in the infected shrimp. Intriguingly, after ruling out the effect of background changes in gut microbiota, we identified 20 infection-discriminatory taxa that could be served as independent variables for accurately (89.4%) diagnosing
V. anguillarum
infection, even at the early infection stage, i.e., 24 hpi. Using a consensus network, we identified several
Vibrio
and
Pseudoalteromonas
taxa that directly antagonized
V. anguillarum
, following the Darwin’s niche theory. This is one of the few attempts to identify gut bioindicators for diagnosing pathogen infection. In addition, the antagonistic commensals of
V. anguillarum
might be the candidate probiotics for preventing AHPND. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1436-2228 1436-2236 |
DOI: | 10.1007/s10126-021-10079-8 |