Prevalence of Enterobacteriaceae spp. and its multidrug-resistant rates in clinical isolates: A two-center cross-sectional study

• Published in: Molecular biology reports Vol. 48; no. 1; pp. 665 - 675
• Main Authors: Mirzaei, Bahman, Babaei, Ryhane, Bazgir, Zahra Norouzi, Goli, Hamid Reza, Keshavarzi, Shima, Amiri, Elham
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 2021; Springer Nature B.V
• Subjects: Amikacin; Ampicillin; Animal Anatomy; Animal Biochemistry; Anti-Bacterial Agents - adverse effects; Anti-Bacterial Agents - therapeutic use; Antibiotic resistance; Antibiotics; Bacteria; Biomedical and Life Sciences; Cephalexin; Clinical isolates; Cross Infection - epidemiology; Cross Infection - genetics; Cross Infection - microbiology; Cross Infection - prevention & control; Cross-sectional studies; Culture media; Drug resistance; Drug Resistance, Multiple, Bacterial - genetics; E coli; Enterobacteriaceae; Enterobacteriaceae - drug effects; Enterobacteriaceae - genetics; Enterobacteriaceae - pathogenicity; Genes; Histology; Hospitals; Humans; Intensive care; Iran - epidemiology; Klebsiella pneumoniae - drug effects; Klebsiella pneumoniae - genetics; Klebsiella pneumoniae - pathogenicity; Laboratories; Life Sciences; Microbial Sensitivity Tests; Molecular biology; Morphology; Mortality; Multidrug resistance; Multidrug resistant organisms; Nalidixic acid; Nosocomial infections; Original Article; RNA polymerase; RpoB protein; Virology; Iran; Susceptibility testing; Hospital-acquired infections; Drug-resistant; Enterobacteriaceae
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-020-06114-x

Enterobacteriaceae spp., owing to their high durability and antibiotic-resistant mechanisms, are described as an eminent part of health treatments in hospital-acquired infections. This study aimed to estimate the prevalence of clinical isolated Enterobacteriaceae spp., and their multidrug-resistant rate in the north of Iran. In this cross-sectional study, over two years (2017–2019), clinical isolates were collected and Enterobacteriaceae spp. were identified using the standard media culture and Analytical Profile Index (API 20E) kit from two centers in the north of Iran. Isolates were confirmed by targeting the rpoB gene. Moreover, the susceptibility patterns of isolates were assessed using disc diffusion methods according to the Clinical Laboratory Standard Institute (CLSI) guidelines. Out of 2645 clinical specimens, 297 (11.2%) were confirmed as Enterobacteriaceae spp. containing Eshershia. coli 93 (31%), Citrobacter freundii 65 (21.9%), Klebsiella pneumoniae 48 (16.2%), Enterobacter spp. 43 (14.5%), and Proteus spp. 23 (7.7%). As much as 8.7% of other spp. Ampicillin (81.1%) and cephalexin (80.9%) have been shown to have the greatest resistant, and nalidixic acid (65%) and amikacin (59.2%) were the most sensitive drugs. Multidrug-resistance (MDR) strains are more isolated in the Burn and Burn intensive care unit (BICU) than other wards. The MDR frequency in Bouali and Zareh hospitals were 65 (49.61%) and 130 (78.31%), respectively. Considering the high isolation rates of MDR Enterobacteriaceae spp., preventive measures need to be taken to remove the mentioned bacteria from hospital wards.