Cationic derivative of electrospun non-woven cellulose-chitosan composite fabrics for immobilization of aminoacylase-I

The chemical modification of cellulose (Cs)-chitosan (Ch)-electrospun non-woven (ESNW) composite fabrics is described. Since the as-spun Cs/Ch-composite ESNW (Cs : Ch = 4 : 6) deforms in water, an insolublilization procedure using an alkaline-ethanol solution was developed to extend the applications...

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Bibliographic Details
Published inTextile research journal Vol. 83; no. 18; pp. 1918 - 1925
Main Authors Devarayan, Kesavan, Miyamoto, Masahiro, Hachisu, Masakazu, Araki, Jun, Periasamy, Viswanathamurthi, Ohkawa, Kousaku
Format Journal Article
LanguageEnglish
Published London, England SAGE Publications 01.11.2013
Sage Publications Ltd
Subjects
Composite materials
Ethanol
Infrared radiation
Nonwoven fabrics
Spinning
Studies
Japan
composites
polysaccharides
Chemical modification
electrospinning
fiber formation
properties
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Summary:The chemical modification of cellulose (Cs)-chitosan (Ch)-electrospun non-woven (ESNW) composite fabrics is described. Since the as-spun Cs/Ch-composite ESNW (Cs : Ch = 4 : 6) deforms in water, an insolublilization procedure using an alkaline-ethanol solution was developed to extend the applications in an aqueous environment. The fine fiber surface was modified with bifunctional isocyanate in order to introduce the -NCO groups on the ESNW surfaces. The -NCO groups were condensed with N,N-(diethyl)ethylene diamine to generate a cationic diethylaminoethyl (DEAE)-ESNW. An enzyme, aminoacylase-I, was immobilized onto the cationic matrix under mild conditions. The immobilized enzyme was subjected to the stereo-specific recognition of Nα-acetyl-methionines. The results suggest that the chemically modified Cs/Ch-ESNW is a useful support matrix for biological catalysts.
ISSN:0040-5175
1746-7748
DOI:10.1177/0040517513478457