Prevalence, Genetic Diversity, and Antibiotic Susceptibility of Cronobacter spp. (Enterobacter sakazakii) Isolated from Sunshik, Its Ingredients and Soils

• Published in: Food science and biotechnology Vol. 20; no. 4; pp. 941 - 948
• Main Authors: Kim, J.B., Gyeonggi-do Institute of Health and Environment, Suwon, Republic of Korea, Park, Y.B., Gyeonggi-do Institute of Health and Environment, Suwon, Republic of Korea, Kang, S.H., Gyeonggi-do Institute of Health and Environment, Suwon, Republic of Korea, Lee, M.J., Gyeonggi-do Institute of Health and Environment, Suwon, Republic of Korea, Kim, K.C., Gyeonggi-do Institute of Health and Environment, Suwon, Republic of Korea, Jeong, H.R., Gyeonggi-do Institute of Health and Environment, Suwon, Republic of Korea, Kim, D.H., Gyeonggi-do Institute of Health and Environment, Suwon, Republic of Korea, Yoon, M.H., Gyeonggi-do Institute of Health and Environment, Suwon, Republic of Korea, Lee, J.B., Gyeonggi-do Institute of Health and Environment, Suwon, Republic of Korea, Oh, D.H., Kangwon National University, Chuncheon, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society of Food Science and Technology 01.08.2011; 한국식품과학회
• Subjects: antibiotic susceptibility; Chemistry; Chemistry and Materials Science; Cronobacter species; Daucus; EMBALSES; Enterobacter sakazakii; Food Science; genotyping; Nutrition; Research Article; RESERVOIR D'EAU; root vegetable; Solanum tuberosum; WATER RESERVOIRS; 식품과학; reservoir; antibiotic susceptibility; species; root vegetable; genotyping
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-011-0130-6

The prevalence, genetic diversity, and antibiotic susceptibility of Cronobacter species (Enterobacter sakazakii) isolated from sunshik products, its ingredients, and root vegetable farm's soils were investigated to analyze main reservoirs and contaminated sources of Cronobacter spp. Cronobacter spp. was isolated from 9 of 15 sunshik products, 26 of 72 its ingredients, and 2 of 39 soils. The root vegetables such as sweet potato and carrot showed higher contamination rate (70%) than the other sunshik ingredients. All isolates showed 929 bp band amplified from 16S rRNA and resistant to ampicillin, amoxicillin/clavulanic acid, and cefazolin. All isolates showed diverse random-amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) band patterns. However, 3 cases of RAPD banding patterns between clinical strains and isolates from sunshik products and root vegetables (yam, carrot) were related with similarities level of 80%. These studies indicated that root vegetables can be an important contamination source of Cronobacter spp. in sunshik products. Thus, the preparation of root vegetables for manufacturing sunshik products used as a weaning diet was handled with more care than the other sunshik ingredients.