Antifungal effects of volatile organic compounds produced by Trichoderma koningiopsis T2 against Verticillium dahliae
Volatile organic compounds (VOCs) produced by microorganisms are considered promising environmental-safety fumigants for controlling soil-borne diseases. Verticillium dahliae , a notorious fungal pathogen, causes economically important wilt diseases in agriculture and forestry industries. Here, we d...
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| Published in | Frontiers in microbiology Vol. 13; p. 1013468 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Frontiers Media S.A
21.09.2022
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| Subjects | |
| Online Access | Get full text |
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| Summary: | Volatile organic compounds (VOCs) produced by microorganisms are considered promising environmental-safety fumigants for controlling soil-borne diseases.
Verticillium dahliae
, a notorious fungal pathogen, causes economically important wilt diseases in agriculture and forestry industries. Here, we determined the antifungal activity of VOCs produced by
Trichoderma koningiopsis
T2. The VOCs from
T. koningiopsis
T2 were trapped by solid-phase microextraction (SPME) and tentatively identified through gas chromatography–mass spectrometry (GC/MS). The microsclerotia formation, cell wall-degrading enzymes and melanin synthesis of
V. dahliae
exposed to the VOC mixtures and selected single standards were examined. The results showed that the VOCs produced by strain T2 significantly inhibited the growth of
V. dahliae
mycelium and reduced the severity of
Verticillium
wilt in tobacco and cotton. Six individual compounds were identified in the volatilome of
T. koningiopsis
T2, and the dominant compounds were 3-octanone, 3-methyl-1-butanol, butanoic acid ethyl ester and 2-hexyl-furan. The VOCs of strain T2 exert a significant inhibitory effect on microsclerotia formation and decreased the activities of pectin lyase and endo-β-1,4-glucanase in
V. dahliae
. VOCs also downregulated the
VdT3HR
,
VdT4HR
, and
VdSCD
genes related to melanin synthesis by 29. 41-, 10. 49-, and 3.11-fold, respectively. Therefore,
T. koningiopsis
T2 has potential as a promising biofumigant for the biocontrol of
Verticillium
wilt disease. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Mario Carlos Saparrat, National University of La Plata, Argentina; Mohammad Djavaheri, Agriculture and Agri-Food Canada (AAFC), Canada Edited by: Zhihui Xu, Nanjing Agricultural University, China This article was submitted to Microbe and Virus Interactions with Plants, a section of the journal Frontiers in Microbiology |
| ISSN: | 1664-302X 1664-302X |
| DOI: | 10.3389/fmicb.2022.1013468 |